ArrayExpress Uploader0mouse344SQ cells with control vector344SQ cells with control vector344SQ cells with control vector344SQ cells with miR-34a overexpression344SQ cells with miR-34a overexpression344SQ cells with miR-34a overexpression8629arrayexpress_sid8The Zeb1 transcriptional repressor plays a key role in metastasis through the down-regulation of genes that are strong inducers of...E-GEOD-38341/profile/8773/arrayexpressuploader16adenocarcinomasgenomelungproteinDec.12, 2014Falsetranscriptional-profiling-of-lung-cancer-cells-oveE-GEOD-38341_A-AFFY-36Transcriptional profiling of lung cancer cells over-expression miR-34a.Nov.24, 2014The Zeb1 transcriptional repressor plays a key role in metastasis through the down-regulation of genes that are strong inducers of epithelial differentiation and inhibitors of stem-ness. Here we report that Zeb1 controls the expression of numerous oncogenic and tumor suppressive microRNAs (miRs). Zeb1 stimulated pro-migratory cytoskeletal processes by down-regulating miR-34a and activated Rho GTPases through Arhgap1, a Cdc42 GTPase activating protein and novel miR-34a target gene. Poor-prognosis human lung adenocarcinomas were highly enriched in a cytoskeletal gene signature activated by miR-34a down-regulation. These findings suggest that Zeb1 regulates a miR network and drives pro-migratory cytoskeletal processes through miR-34a. Total RNA was extracted from primary tumors from mice injected with 344SQ-vector and -miR-34a cells, and then hybridized to Affymetrix GeneChip Mouse Genome 430 2.0 array. Determination of differentially expressed genes was carried out.http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-38341http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-38341/samples/