{"owner": "ArrayExpress Uploader", "pop_total": 0, "species": "mouse", "factors": [{"GSM940327": {"CELL TYPE": "344SQ cells with control vector"}}, {"GSM940327": {"CELL TYPE": "344SQ cells with control vector"}}, {"GSM940327": {"CELL TYPE": "344SQ cells with control vector"}}, {"GSM940330": {"CELL TYPE": "344SQ cells with miR-34a overexpression"}}, {"GSM940330": {"CELL TYPE": "344SQ cells with miR-34a overexpression"}}, {"GSM940330": {"CELL TYPE": "344SQ cells with miR-34a overexpression"}}], "id": 8629, "ownerprofile_id": "arrayexpress_sid", "platform": 8, "summary_wrapped": "The Zeb1 transcriptional repressor plays a key role in metastasis through the down-regulation of genes that are strong inducers of...", "geo_gse_id": "E-GEOD-38341", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 1, "sample_count": 6, "tags": ["adenocarcinomas", "genome", "lung", "protein"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_gds_id": "", "slug": "transcriptional-profiling-of-lung-cancer-cells-ove", "geo_id_plat": "E-GEOD-38341_A-AFFY-36", "name": "Transcriptional profiling of lung cancer cells over-expression miR-34a.", "created": "Nov.24, 2014", "summary": "The Zeb1 transcriptional repressor plays a key role in metastasis through the down-regulation of genes that are strong inducers of epithelial differentiation and inhibitors of stem-ness. Here we report that Zeb1 controls the expression of numerous oncogenic and tumor suppressive microRNAs (miRs). Zeb1 stimulated pro-migratory cytoskeletal processes by down-regulating miR-34a and activated Rho GTPases through Arhgap1, a Cdc42 GTPase activating protein and novel miR-34a target gene. Poor-prognosis human lung adenocarcinomas were highly enriched in a cytoskeletal gene signature activated by miR-34a down-regulation. These findings suggest that Zeb1 regulates a miR network and drives pro-migratory cytoskeletal processes through miR-34a. Total RNA was extracted from primary tumors from mice injected with 344SQ-vector and -miR-34a cells, and then hybridized to Affymetrix GeneChip Mouse Genome 430 2.0 array. Determination of differentially expressed genes was carried out.", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-38341", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-38341/samples/"}