{"owner": "ArrayExpress Uploader", "pop_total": 0, "id": 6900, "factors": [{"GSM936877": {"genotype": "wild type genotype"}}, {"GSM936877": {"genotype": "wild type genotype"}}, {"GSM936877": {"genotype": "wild type genotype"}}, {"GSM936877": {"genotype": "wild type genotype"}}, {"GSM936877": {"genotype": "wild type genotype"}}, {"GSM936877": {"genotype": "wild type genotype"}}, {"GSM936877": {"genotype": "wild type genotype"}}, {"GSM936877": {"genotype": "wild type genotype"}}, {"GSM936885": {"genotype": "R6/2::HDAC4het"}}, {"GSM936885": {"genotype": "R6/2::HDAC4het"}}, {"GSM936885": {"genotype": "R6/2::HDAC4het"}}, {"GSM936885": {"genotype": "R6/2::HDAC4het"}}, {"GSM936885": {"genotype": "R6/2::HDAC4het"}}, {"GSM936885": {"genotype": "R6/2::HDAC4het"}}, {"GSM936885": {"genotype": "R6/2::HDAC4het"}}, {"GSM936885": {"genotype": "R6/2::HDAC4het"}}, {"GSM936893": {"genotype": "R6/2"}}, {"GSM936893": {"genotype": "R6/2"}}, {"GSM936893": {"genotype": "R6/2"}}, {"GSM936893": {"genotype": "R6/2"}}, {"GSM936893": {"genotype": "R6/2"}}, {"GSM936893": {"genotype": "R6/2"}}, {"GSM936893": {"genotype": "R6/2"}}, {"GSM936893": {"genotype": "R6/2"}}, {"GSM936893": {"genotype": "R6/2"}}, {"GSM936902": {"genotype": "HDAC4het"}}, {"GSM936902": {"genotype": "HDAC4het"}}, {"GSM936902": {"genotype": "HDAC4het"}}, {"GSM936902": {"genotype": "HDAC4het"}}, {"GSM936902": {"genotype": "HDAC4het"}}, {"GSM936902": {"genotype": "HDAC4het"}}, {"GSM936902": {"genotype": "HDAC4het"}}, {"GSM936902": {"genotype": "HDAC4het"}}, {"GSM936902": {"genotype": "HDAC4het"}}], "ownerprofile_id": "arrayexpress_sid", "platform": 6, "summary_wrapped": "[u\"Histone deacetylase (HDAC) 4 is a transcriptional repressor that contains a glutamine rich domain. We hypothesised that it may be...", "pubmed_id": 24302884, "geo_gse_id": "E-GEOD-38219", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 1, "sample_count": 34, "tags": ["disease", "glutamine", "histone", "huntington's disease", "protein"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_gds_id": "", "slug": "gene-expression-data-from-cortex-of-15-week-old-wi", "geo_id_plat": "E-GEOD-38219_A-AFFY-45", "name": "Gene expression data from cortex of 15 week old wild type, R6/2, HDAC4het and R6/2::HDAC4het mice", "created": "Nov.12, 2014", "summary": "[u\"Histone deacetylase (HDAC) 4 is a transcriptional repressor that contains a glutamine rich domain. We hypothesised that it may be involved in the molecular pathogenesis of Huntington's disease (HD), a protein folding neurodegenerative disorder caused by an aggregation-prone polyglutamine expansion and transcriptional dysregulation.   We found that HDAC4 interacts with huntingtin in a polyglutamine-length dependent manner and co-localises with cytoplasmic inclusions. We show that HDAC4 reduction delayed cytoplasmic aggregate formation, restored Bdnf transcript levels and rescued neuronal and cortico-striatal synaptic function in HD mouse models. This was accompanied by an improvement in motor co-ordination, neurological phenotypes and increased lifespan. Surprisingly, HDAC4 reduction had no effect on global transcriptional dysfunction and did not modulate nuclear huntingtin aggregation.     Our results define a crucial role for cytoplasmic aggregation in the molecular pathology of HD. HDAC4 reduction presents a novel strategy for targeting huntingtin aggregation which may be amenable to small molecule therapeutics. mRNA expression analysis was performed by microarray in 15 weeks old WT (n=8), R6/2 (n=9), HDAC4het (n=8) and Double R6/2::HDAC4het (n=9) mice. Microarray quality control was performed using the software package provided on RACE (\", {u'a': {u'href': u'http://race.unil.ch', u'target': u'_blank', u'$': u'http://race.unil.ch'}}, u').']", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-38219", "species": "mouse", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-38219/samples/"}