6ArrayExpress Uploader06892wild type Ikaros (HA-Ikaros-IRES-GFP)not specifiedwild type Ikaros (HA-Ikaros-IRES-GFP)not specifiedIkaros mutant 159A (HA-159A Ikaros-IRES-GFP)not specifiedIkaros mutant 159A (HA-159A Ikaros-IRES-GFP)not specifiedarrayexpress_sidhttp://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-38110Ikaros family DNA binding proteins are critical regulators of B cell development. To identify Ikaros-regulated genes in pre-B cells we...23303821/profile/8773/arrayexpressuploader24celllineDec.12, 2014Falsegene-expression-in-mouse-pre-b-cells-transduced-wiE-GEOD-38110_A-AFFY-45Gene expression in mouse pre-B cells transduced with Ikaros.Nov.12, 2014Ikaros family DNA binding proteins are critical regulators of B cell development. To identify Ikaros-regulated genes in pre-B cells we performed gene expression studies at enhanced temporal resolution. We used retroviral gene transfer to express Ikaros proteins in the pre-B cell line B3. Total RNA from 2 biological replicates of B3 cells transduced with wild type Ikaros (HA-Ikaros-IRES-GFP) and DNA binding-deficient Ikaros mutant 159A (HA-159A Ikaros-IRES-GFP) was isolated 48h after infection. To increase the temporal resolution of Ikaros-regulated gene expression we transduced B3 cells with inducible Ikaros (HA-Ikaros-ERt2-IRES-GFP) and isolated total RNA from 3 biological replicates after 2 h and 6 h of exposure to 4-hydroxytamoxifen. Vector transduced B3 cells (ERt2-IRES-GFP) treated with 4-hydroxytamoxifen were used as controls.E-GEOD-38110mousehttp://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-38110/samples/