{"platform": 6, "owner": "ArrayExpress Uploader", "pop_total": 0, "id": 6892, "factors": [{"GSM934758": {"TRANSDUCED WITH": "wild type Ikaros (HA-Ikaros-IRES-GFP)", "TREATED WITH": "not specified"}}, {"GSM934758": {"TRANSDUCED WITH": "wild type Ikaros (HA-Ikaros-IRES-GFP)", "TREATED WITH": "not specified"}}, {"GSM934760": {"TRANSDUCED WITH": "Ikaros mutant 159A (HA-159A Ikaros-IRES-GFP)", "TREATED WITH": "not specified"}}, {"GSM934760": {"TRANSDUCED WITH": "Ikaros mutant 159A (HA-159A Ikaros-IRES-GFP)", "TREATED WITH": "not specified"}}], "ownerprofile_id": "arrayexpress_sid", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-38110", "summary_wrapped": "Ikaros family DNA binding proteins are critical regulators of B cell development. To identify Ikaros-regulated genes in pre-B cells we...", "pubmed_id": 23303821, "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 2, "sample_count": 4, "tags": ["cell", "line"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_gds_id": "", "slug": "gene-expression-in-mouse-pre-b-cells-transduced-wi", "geo_id_plat": "E-GEOD-38110_A-AFFY-45", "name": "Gene expression in mouse pre-B cells transduced with Ikaros.", "created": "Nov.12, 2014", "summary": "Ikaros family DNA binding proteins are critical regulators of B cell development. To identify Ikaros-regulated genes in pre-B cells we performed gene expression studies at enhanced temporal resolution. We used retroviral gene transfer to express Ikaros proteins in the pre-B cell line B3. Total RNA from 2 biological replicates of B3 cells transduced with wild type Ikaros (HA-Ikaros-IRES-GFP) and DNA binding-deficient Ikaros mutant 159A (HA-159A Ikaros-IRES-GFP) was isolated 48h after infection. To increase the temporal resolution of Ikaros-regulated gene expression we transduced B3 cells with inducible Ikaros (HA-Ikaros-ERt2-IRES-GFP) and isolated  total RNA from 3 biological replicates after 2 h and 6 h of exposure to 4-hydroxytamoxifen. Vector transduced B3 cells (ERt2-IRES-GFP)  treated with 4-hydroxytamoxifen were used as controls.", "geo_gse_id": "E-GEOD-38110", "species": "mouse", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-38110/samples/"}