ArrayExpress Uploader0mouseRAG-2-deficientRAG-2-deficientRAG-2-deficientArtemis-deficientArtemis-deficientArtemis-deficient6887arrayexpress_sid6The objective is to identify genes that are differentially expressed following the introduction of DNA double-strand breaks (DSBs) by the...E-GEOD-38044/profile/8773/arrayexpressuploader16cellgenomeDec.12, 2014Falsegene-activation-by-rag-mediated-dna-double-strandE-GEOD-38044_A-AFFY-45Gene activation by Rag-mediated DNA double-strand breaks in G1-phase murine pre-B cellsNov.12, 2014The objective is to identify genes that are differentially expressed following the introduction of DNA double-strand breaks (DSBs) by the Rag proteins in murine pre-B cells. Cells lacking Artemis are used since the Rag-induced DSBs will not be repaired, and thus, will provide a continuous stimulus to the cell. Murine v-abl-transformed pre-B cells were treated with 3 uM STI571 for 48 hours. Cell types included RAG-2-deficient (3 biological replicates) and Artemis-deficient (3 biological replicates) G1-phase pre-B cells. Each sample was hybridized once using Affymetrix Mouse Genome 2.0 GeneChip arrays (Mouse 430 v2, Affymetrix, Santa Clara, CA). Data were analyzed using the RAG-2-deficient samples as the controls.http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-38044http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-38044/samples/