{"platform": 8, "owner": "ArrayExpress Uploader", "pop_total": 0, "species": "mouse", "factors": [{"GSM929694": {"TBX1 GENOTYPE": "Tbx1+/+"}}, {"GSM929694": {"TBX1 GENOTYPE": "Tbx1+/+"}}, {"GSM929694": {"TBX1 GENOTYPE": "Tbx1+/+"}}, {"GSM929697": {"TBX1 GENOTYPE": "Tbx1+/-"}}, {"GSM929697": {"TBX1 GENOTYPE": "Tbx1+/-"}}, {"GSM929697": {"TBX1 GENOTYPE": "Tbx1+/-"}}, {"GSM929700": {"TBX1 GENOTYPE": "Tbx1-/-"}}, {"GSM929700": {"TBX1 GENOTYPE": "Tbx1-/-"}}, {"GSM929700": {"TBX1 GENOTYPE": "Tbx1-/-"}}], "id": 8625, "ownerprofile_id": "arrayexpress_sid", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-37904", "summary_wrapped": "To assess the influence of Tbx1 on gene expression profile within the developing palate we performed a microarray screen using RNA...", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 1, "sample_count": 9, "tags": ["palate"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_gds_id": "", "slug": "expression-profiling-of-palatal-shelves-in-tbx1-mu", "geo_id_plat": "E-GEOD-37904_A-AFFY-36", "name": "Expression profiling of palatal shelves in Tbx1 mutant mice", "created": "Nov.24, 2014", "summary": "To assess the influence of Tbx1 on gene expression profile within the developing palate we performed a microarray screen using RNA isolated from dissected secondary palate shelves of E13.5 wild type, Tbx1+/- and Tbx1-/- mice. Significant differences were identified between genotypes, with a total of 67 genes demonstrating at least a 2-fold change (p<0.05) in expression. These were clustered into 5 groups, including those downregulated in mutant compared to wild type and heterozygote (n=36); those progressively downregulated from wild type to mutant (n=12); those upregulated in heterozygote and downregulated in mutant compared to wild type (n=2); those progressively upregulated from wild type to mutant (n=12) and those downregulated in heterozygote and upregulated in mutant compared to wild type (n=5). High-throughput real time quantitative RT-PCR confirmed a total of 18 genes significantly changed between wild type and mutant and 24 between heterozygote and mutant. Amongst these, 15 were present in both groups and all except 1 were downregulated in the mutant. There were no significant differences in gene expression between wild type and heterozygous palatal shelves. Secondary palatal shelf pairs were carefully microdissected from E13.5 Tbx1+/+; Tbx1+/- and Tbx1-/- embryos (3 embryos per genotype as biological replicates). RNA was extracted from each pooled shelf pair generating nine RNA samples in total, each one analysed using a single microarray.", "geo_gse_id": "E-GEOD-37904", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-37904/samples/"}