{"owner": "ArrayExpress Uploader", "pop_total": 0, "id": 6875, "factors": [{"GSM928277": {"FACS SORTED FOR": "dsRed2+ and TTP-GFP-", "VARIATION": "MSCV-I-dsRed2+; MSCV-TTP-I-GFP-"}}, {"GSM928277": {"FACS SORTED FOR": "dsRed2+ and TTP-GFP-", "VARIATION": "MSCV-I-dsRed2+; MSCV-TTP-I-GFP-"}}, {"GSM928277": {"FACS SORTED FOR": "dsRed2+ and TTP-GFP-", "VARIATION": "MSCV-I-dsRed2+; MSCV-TTP-I-GFP-"}}, {"GSM928280": {"FACS SORTED FOR": "dsRed2+ and TTP-GFP+", "VARIATION": "MSCV-I-dsRed2+; MSCV-TTP-I-GFP+"}}, {"GSM928280": {"FACS SORTED FOR": "dsRed2+ and TTP-GFP+", "VARIATION": "MSCV-I-dsRed2+; MSCV-TTP-I-GFP+"}}, {"GSM928280": {"FACS SORTED FOR": "dsRed2+ and TTP-GFP+", "VARIATION": "MSCV-I-dsRed2+; MSCV-TTP-I-GFP+"}}], "ownerprofile_id": "arrayexpress_sid", "platform": 6, "summary_wrapped": "Myc oncoproteins directly regulate transcription by binding to target genes, yet this only explains a fraction of the genes affected by...", "pubmed_id": 22863009, "geo_gse_id": "E-GEOD-37791", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 2, "sample_count": 6, "tags": ["cancer", "lymphoma"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_gds_id": "", "slug": "tristetraprolin-is-a-tumor-suppressor-that-impai-3", "geo_id_plat": "E-GEOD-37791_A-AFFY-45", "name": "Tristetraprolin is a tumor suppressor that impairs Myc-induced lymphoma and abolishes the malignant state [retrovirally infected ex vivo lymphoma]", "created": "Nov.12, 2014", "summary": "Myc oncoproteins directly regulate transcription by binding to target genes, yet this only explains a fraction of the genes affected by Myc. mRNA turnover is controlled via AU-binding proteins (AUBPs) that recognize AU-rich elements (AREs) found within many transcripts. Analyses of precancerous and malignant Myc-expressing B cells revealed that Myc regulates hundreds of ARE-containing (ARED) genes and select AUBPs. Notably, Myc directly suppresses transcription of Tristetraprolin (TTP/ZFP36), an mRNA-destabilizing AUBP, and this circuit is also operational during B lymphopoiesis and IL7 signaling. Importantly, TTP suppression is a hallmark of cancers with MYC involvement, and restoring TTP impairs Myc-induced lymphomagenesis and abolishes maintenance of the malignant state. Further, there is a selection for TTP loss in malignancy; thus, TTP functions as a tumor suppressor. Finally, Myc/TTP-directed control of select cancer-associated ARED genes is disabled during lymphomagenesis. Thus, Myc targets AUBPs to regulate ARED genes that control tumorigenesis. Ex vivo E\u03bc-Myc lymphoma cells were infected with MSCV-I-dsRed2 (RFP) and FACS sorted for dsRed2+ cells. These cells were then infected with MSCV-TTP-I-GFP and FACS sorted for GFP expression (TTP-GFP- or TTP-GFP+). These cells were used for subsequent RNA purification, labeling and hybridization to MOE430 2.0 Affymetrix arrays.", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-37791", "species": "mouse", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-37791/samples/"}