{"owner": "ArrayExpress Uploader", "pop_total": 0, "species": "mouse", "factors": [{"GSM928133": {"CELL TYPE": "ES cell", "GENOTYPE": "Wild-type"}}, {"GSM928133": {"CELL TYPE": "ES cell", "GENOTYPE": "Wild-type"}}, {"GSM928135": {"CELL TYPE": "ES cell", "GENOTYPE": "Men1-KO"}}, {"GSM928135": {"CELL TYPE": "ES cell", "GENOTYPE": "Men1-KO"}}, {"GSM928137": {"CELL TYPE": "ES cells were differentiated into pancreatic islet-like endocrine cells (PILECs)", "GENOTYPE": "Wild-type"}}, {"GSM928137": {"CELL TYPE": "ES cells were differentiated into pancreatic islet-like endocrine cells (PILECs)", "GENOTYPE": "Wild-type"}}, {"GSM928139": {"CELL TYPE": "ES cells were differentiated into pancreatic islet-like endocrine cells (PILECs)", "GENOTYPE": "Men1-KO"}}, {"GSM928139": {"CELL TYPE": "ES cells were differentiated into pancreatic islet-like endocrine cells (PILECs)", "GENOTYPE": "Men1-KO"}}], "id": 6872, "ownerprofile_id": "arrayexpress_sid", "platform": 6, "summary_wrapped": "Inactivating mutations in the MEN1 gene predisposing to the multiple endocrine neoplasia type 1 (MEN1) syndrome can also cause sporadic...", "geo_gse_id": "E-GEOD-37775", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 2, "sample_count": 8, "tags": ["cell", "genome", "histone", "histone h3", "lysine", "multiple endocrine neoplasia", "multiple endocrine neoplasia type 1", "pancreatic islet", "syndrome"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_gds_id": "", "slug": "genome-wide-characterization-of-menin-dependent-h3", "geo_id_plat": "E-GEOD-37775_A-AFFY-45", "name": "Genome-wide characterization of menin-dependent H3K4me3 reveals a specific role for menin in the regulation of genes implicated in MEN1-like tumors (mRNA)", "created": "Nov.12, 2014", "summary": "Inactivating mutations in the MEN1 gene predisposing to the multiple endocrine neoplasia type 1 (MEN1) syndrome can also cause sporadic pancreatic endocrine tumors. MEN1 encodes menin, a subunit of MLL1/MLL2-containing histone methyltransferase complexes that trimethylate histone H3 at lysine 4 (H3K4me3). The importance of menin-dependent H3K4me3 in normal and transformed pancreatic endocrine cells is unclear. To study the role of menin-dependent H3K4me3, we performed in vitro differentiation of wild-type as well as menin-null mouse embryonic stem cells (mESCs) into pancreatic islet-like endocrine cells (PILECs). Gene expression analysis and genome-wide H3K4me3 ChIP-Seq profiling in wild-type and menin-null mESCs and PILECs revealed menin-dependent H3K4me3 at the imprinted Dlk1-Meg3 locus in mESCs, and all four Hox loci in differentiated PILECs. Specific and significant loss of H3K4me3 and gene expression was observed for genes within the imprinted Dlk1-Meg3 locus in menin-null mESCs and the Hox loci in menin-null PILECs. Given that the reduced expression of genes within the DLK1-MEG3 locus and the HOX loci is associated with MEN1-like sporadic tumors, our data suggests a possible role for menin-dependent H3K4me3 at these genes in the initiation and progression of sporadic pancreatic endocrine tumors. Furthermore, our investigation also demonstrates that menin-null mESCs can be differentiated in vitro into islet-like endocrine cells, underscoring the utility of menin-null mESC-derived specialized cell types for genome-wide high-throughput studies. Genome-wide mapping of H3K4me3 and microarray gene expression profiling in TC-1 wild-type (WT) mESCs, menin-null (Men1-ko) mESCs (3.2N), pancreatic islet-like endocrine cells (PILECs) derived from WT mESCs, and PILECs derived from Men1-ko mESCs.", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-37775", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-37775/samples/"}