ArrayExpress Uploader06868controlEtv2 overexpressioncontrolEtv2 overexpressioncontrolEtv2 overexpressionarrayexpress_sid6During embryogenesis, the endothelial and the hematopoietic lineages first appear during gastrulation in the blood island of the yolk...22628281E-GEOD-37658/profile/8773/arrayexpressuploader16cellembryolineyolk sacDec.12, 2014Falsegene-expression-analysis-of-inducible-es-cells-oveE-GEOD-37658_A-AFFY-45Gene expression analysis of inducible ES cells overexpressing Etv2 (induced for 12 hours at day 3 of differentiation)Nov.12, 2014During embryogenesis, the endothelial and the hematopoietic lineages first appear during gastrulation in the blood island of the yolk sac. We have previously reported that an Ets variant gene 2 (Etv2/ER71) mutant embryo lacks hematopoietic and endothelial lineages, however, the precise roles of Etv2 in yolk sac development remains unclear. We carried out a transcriptome analysis using an ES cell line that can inducibly express Etv2. Cells were induced for 12 hours at day 3 of differentiation. An ES cell line (dervied from mouse of background strain 129P2/Ola) with a doxycycline inducible expression casette of Etv2 (ER71 A2Lox.cre) was differentiated in hanging drops. At 3 days of differentiation, cells were treated with doxycycline for 12 hours, and subsequently Flk1(+) cells were sorted. Experiment was done in triplicate and untreated cells were used for control.http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-37658mousehttp://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-37658/samples/