{"owner": "ArrayExpress Uploader", "pop_total": 0, "id": 6868, "factors": [{"GSM925107": {"VARIATION": "control"}}, {"GSM925108": {"VARIATION": "Etv2 overexpression"}}, {"GSM925107": {"VARIATION": "control"}}, {"GSM925108": {"VARIATION": "Etv2 overexpression"}}, {"GSM925107": {"VARIATION": "control"}}, {"GSM925108": {"VARIATION": "Etv2 overexpression"}}], "ownerprofile_id": "arrayexpress_sid", "platform": 6, "summary_wrapped": "During embryogenesis, the endothelial and the hematopoietic lineages first appear during gastrulation in the blood island of the yolk...", "pubmed_id": 22628281, "geo_gse_id": "E-GEOD-37658", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 1, "sample_count": 6, "tags": ["cell", "embryo", "line", "yolk sac"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_gds_id": "", "slug": "gene-expression-analysis-of-inducible-es-cells-ove", "geo_id_plat": "E-GEOD-37658_A-AFFY-45", "name": "Gene expression analysis of inducible ES cells overexpressing Etv2 (induced for 12 hours at day 3 of differentiation)", "created": "Nov.12, 2014", "summary": "During embryogenesis, the endothelial and the hematopoietic lineages first appear during gastrulation in the blood island of the yolk sac. We have previously reported that an Ets variant gene 2 (Etv2/ER71) mutant embryo lacks hematopoietic and endothelial lineages, however, the precise roles of Etv2 in yolk sac development remains unclear. We carried out a transcriptome analysis using an ES cell line that can inducibly express Etv2. Cells were induced  for 12 hours at day 3 of differentiation. An ES cell line (dervied from mouse of background strain 129P2/Ola) with a doxycycline inducible expression casette of Etv2 (ER71 A2Lox.cre) was differentiated in hanging drops. At 3 days of differentiation, cells were treated with doxycycline for 12 hours, and subsequently Flk1(+) cells were sorted. Experiment was done in triplicate and untreated cells were used for control.", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-37658", "species": "mouse", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-37658/samples/"}