ArrayExpress Uploader0humancontrol PMNs0 hrcontrol PMNs0 hrcontrol PMNs0 hrcontrol PMNs0 hrtest PMNs0 hrtest PMNs0 hrtest PMNs0 hrtest PMNs0 hrcontrol PMNs3 hrcontrol PMNs3 hrcontrol PMNs3 hrcontrol PMNs3 hrtest PMNs3 hrtest PMNs3 hrtest PMNs3 hrtest PMNs3 hrcontrol PMNs6 hrcontrol PMNs6 hrcontrol PMNs6 hrcontrol PMNs6 hrtest PMNs6 hrtest PMNs6 hrtest PMNs6 hrtest PMNs6 hrcontrol PMNs12 hrcontrol PMNs12 hrcontrol PMNs12 hrcontrol PMNs12 hrtest PMNs12 hrtest PMNs12 hrtest PMNs12 hrtest PMNs12 hrcontrol PMNs24 hrcontrol PMNs24 hrcontrol PMNs24 hrcontrol PMNs24 hrtest PMNs24 hrtest PMNs24 hrtest PMNs24 hrtest PMNs24 hrcontrol PMNs48 hrcontrol PMNs48 hrcontrol PMNs48 hrcontrol PMNs48 hrtest PMNs48 hrtest PMNs48 hrtest PMNs48 hrtest PMNs48 hr4468arrayexpress_sid4We demonstrated recently that both constitutive and FAS-triggered apoptosis of human neutrophils are profoundly impaired by Francisella...E-GEOD-37416/profile/8773/arrayexpressuploader248neutrophilDec.12, 2014FalseE-GEOD-37416_A-AFFY-44gene-expression-data-from-f-tularensis-exposed-neuGene expression data from F. tularensis-exposed neutrophilsSep.19, 2014We demonstrated recently that both constitutive and FAS-triggered apoptosis of human neutrophils are profoundly impaired by Francisella tularensis, but how this is achieved is largely unknown. To test the hypothesis that changes in neutrophil gene expression contribute to this phenotype, we used human oligonucleotide microarrays to identify differentially regulated genes in cells infected with F. tularensis strain LVS compared with uninfected controls. In order to examine the effect of F. tularensis on the neutrophil transcriptome, we performed microarray expression analysis on human neutrophils treated with F. tularensis subsp. holarctica live vaccine strain (LVS). Polymorphonuclear leukocytes (PMNs) were isolated from the blood of healthy donors. Control and F. tularensis-exposed PMNs were incubated at 37C for 0, 3, 6, 12, 24, and 48 hours.http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-37416http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-37416/samples/