{"owner": "ArrayExpress Uploader", "pop_total": 0, "species": "human", "factors": [{"GSM918100": {"TRANSFECTION": "A673sh transiently with the pRS-sh-FOXO1 plasmid targeting endogenous FOXO1wt + doxycycline for 48h post transfection"}}, {"GSM918099": {"TRANSFECTION": "A673sh transiently with the pRS-sh-FOXO1 plasmid targeting endogenous FOXO1wt"}}, {"GSM918098": {"TRANSFECTION": "A673sh transiently with the pRS-sh-scrambled plasmid + doxycycline for 48h post transfection"}}, {"GSM918097": {"TRANSFECTION": "A673sh transiently with the pRS-sh-scrambled plasmid"}}, {"GSM918100": {"TRANSFECTION": "A673sh transiently with the pRS-sh-FOXO1 plasmid targeting endogenous FOXO1wt + doxycycline for 48h post transfection"}}, {"GSM918099": {"TRANSFECTION": "A673sh transiently with the pRS-sh-FOXO1 plasmid targeting endogenous FOXO1wt"}}, {"GSM918098": {"TRANSFECTION": "A673sh transiently with the pRS-sh-scrambled plasmid + doxycycline for 48h post transfection"}}, {"GSM918097": {"TRANSFECTION": "A673sh transiently with the pRS-sh-scrambled plasmid"}}, {"GSM918092": {"TRANSFECTION": "A673sh transiently with the pCDNA3-based FLAG-FOXO1-S249A/S298A plasmid"}}, {"GSM91809": {"TRANSFECTION": "A673sh transiently with the pCDNA3-based FLAG-FOXO1-AAA plasmid"}}, {"GSM918090": {"TRANSFECTION": "with the empty vector control (pCDNA3)"}}], "id": 2373, "ownerprofile_id": "arrayexpress_sid", "platform": 4, "summary_wrapped": "Transient transfection of a Ewing's Sarcoma cell line expressing type I EWS-FLI1 fusion and doxycycline-inducible short hairpin RNA...", "geo_gse_id": "E-GEOD-37409", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 1, "sample_count": 11, "tags": ["cell", "line", "sarcoma"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_gds_id": "", "slug": "re-activation-of-ews-fli1-suppressed-foxo1-express", "geo_id_plat": "E-GEOD-37409_A-AFFY-44", "name": "Re-activation of EWS-FLI1 suppressed FOXO1 expression as a novel therapeutic strategy for Ewing\u00b4s sarcoma", "created": "Jul.12, 2014", "summary": "Transient transfection of a Ewing's Sarcoma cell line expressing type I EWS-FLI1 fusion and doxycycline-inducible short hairpin RNA against EWS-FLI1 (A673sh) In total, 7 samples were analysed: empty vector control and two nuclear directed AKT- and CDK2- phosphorylation resistant FOXO1 versions as well as  sh-scrambled and sh-FOXO1, either in the presence (w.o. Doxy.) or absence of EWS-FLI1 (+ Doxy.) each 2 replicates", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-37409", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-37409/samples/"}