Dataset: Re-activation of EWS-FLI1 suppressed FOXO1 expression as a novel therapeutic strategy for Ewing´s sarcoma
Transient transfection of a Ewing's Sarcoma cell line expressing type I EWS-FLI1 fusion and doxycycline-inducible short hairpin RNA...
Transient transfection of a Ewing's Sarcoma cell line expressing type I EWS-FLI1 fusion and doxycycline-inducible short hairpin RNA against EWS-FLI1 (A673sh) In total, 7 samples were analysed: empty vector control and two nuclear directed AKT- and CDK2- phosphorylation resistant FOXO1 versions as well as sh-scrambled and sh-FOXO1, either in the presence (w.o. Doxy.) or absence of EWS-FLI1 (+ Doxy.) each 2 replicates
- Species:
- human
- Samples:
- 11
- Source:
- E-GEOD-37409
- Updated:
- Dec.12, 2014
- Registered:
- Jul.12, 2014
Factors:
(via ArrayExpress)
| Sample | TRANSFECTION |
|---|---|
| GSM918100 | A673sh transiently with the pRS-sh-FOXO1 plasmid targeting endogenous FOXO1wt + doxycycline for 48h post transfection |
| GSM918099 | A673sh transiently with the pRS-sh-FOXO1 plasmid targeting endogenous FOXO1wt |
| GSM918098 | A673sh transiently with the pRS-sh-scrambled plasmid + doxycycline for 48h post transfection |
| GSM918097 | A673sh transiently with the pRS-sh-scrambled plasmid |
| GSM918100 | A673sh transiently with the pRS-sh-FOXO1 plasmid targeting endogenous FOXO1wt + doxycycline for 48h post transfection |
| GSM918099 | A673sh transiently with the pRS-sh-FOXO1 plasmid targeting endogenous FOXO1wt |
| GSM918098 | A673sh transiently with the pRS-sh-scrambled plasmid + doxycycline for 48h post transfection |
| GSM918097 | A673sh transiently with the pRS-sh-scrambled plasmid |
| GSM918092 | A673sh transiently with the pCDNA3-based FLAG-FOXO1-S249A/S298A plasmid |
| GSM91809 | A673sh transiently with the pCDNA3-based FLAG-FOXO1-AAA plasmid |
| GSM918090 | with the empty vector control (pCDNA3) |