{"owner": "ArrayExpress Uploader", "ownerprofile_id": "arrayexpress_sid", "species": "human", "factors": [{"GSM905363": {"VARIATION": "2.5 million copies of EGFRvIII receptor per cell"}}, {"GSM905363": {"VARIATION": "2.5 million copies of EGFRvIII receptor per cell"}}, {"GSM90536": {"VARIATION": "2 million copies of EGFRvIII receptor per cell"}}, {"GSM90536": {"VARIATION": "2 million copies of EGFRvIII receptor per cell"}}, {"GSM905359": {"VARIATION": "1.5 million copies of EGFRvIII receptor per cell"}}, {"GSM905359": {"VARIATION": "1.5 million copies of EGFRvIII receptor per cell"}}, {"GSM905357": {"VARIATION": "kinase dead EGFRvIII"}}, {"GSM905357": {"VARIATION": "kinase dead EGFRvIII"}}], "id": 2610, "pop_total": 0, "platform": 4, "summary_wrapped": "EGFRvIII is the most common deletion mutant of EGFR in human cancer and its levels are highly correlated with poor prognosis in GBM.  The...", "geo_gse_id": "E-GEOD-36901", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 1, "sample_count": 8, "tags": ["cancer", "cell", "gbm"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_id_plat": "E-GEOD-36901_A-AFFY-44", "slug": "expression-data-from-u87mg-cells-expressing-egfrvi", "geo_gds_id": "", "name": "Expression data from U87MG cells expressing EGFRvIII", "created": "Jul.12, 2014", "summary": "EGFRvIII is the most common deletion mutant of EGFR in human cancer and its levels are highly correlated with poor prognosis in GBM.  The deletion of exons 2-7 removes most of the extracellular ligand binding domain, so it is unable to bind EGF or other EGFR-binding ligands.  Nevertheless, the mutant receptor is constitutively phosphorylated, and is capable of activating downstream signaling pathways at a low level. To comprehensively identify the downstream signaling consequences of the EGFRvIII, we incorporated phosphoproteomic, transcription profiling and DNase-Seq data from U87MG glioblastoma cells expressing titrated levels of this mutant receptor. Total RNA were extracted from U87MG cells engineered to expressed different levels of EGFRvIII: medium (U87M; 1.5 million copies of EGFRvIII receptor per cell), high (U87H; 2 million copies per cell), super-high (U87SH; 2.5 million copies per cell), and kinase-dead EGFRvIII (U87DK; 2 million copies of kinase dead EGFRvIII per cell).  RNA was hybridized to Affymetrix microarrays.", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-36901", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-36901/samples/"}