{"owner": "ArrayExpress Uploader", "ownerprofile_id": "arrayexpress_sid", "species": "mouse", "factors": [{"GSM900982 1": {}}, {"GSM901053 1": {}}, {"GSM901054 1": {}}, {"GSM901055 1": {}}, {"GSM901056 1": {}}, {"GSM901057 1": {}}, {"GSM901058 1": {}}, {"GSM901059 1": {}}, {"GSM901060 1": {}}, {"GSM901061 1": {}}, {"GSM901062 1": {}}, {"GSM901063 1": {}}, {"GSM901064 1": {}}, {"GSM901065 1": {}}, {"GSM901066 1": {}}, {"GSM901067 1": {}}, {"GSM901068 1": {}}, {"GSM901087 1": {}}], "id": 6833, "pop_total": 0, "platform": 6, "summary_wrapped": "Dietary interventions are effective ways to extend or shorten lifespan. By examining midlife hepatic gene expressions in mice under...", "geo_gse_id": "E-GEOD-36838", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 0, "sample_count": 18, "tags": ["genome"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_gds_id": "", "slug": "expression-profiling-of-the-effect-of-high-fat-die", "geo_id_plat": "E-GEOD-36838_A-AFFY-45", "name": "Expression profiling of the effect of high-fat diet, low-fat diet, CR and exercise on mice liver", "created": "Nov.12, 2014", "summary": "Dietary interventions are effective ways to extend or shorten lifespan. By examining midlife hepatic gene expressions in mice under different dietary conditions, which resulted in different lifespans and aging-related phenotypes, we were able to identify genes and pathways that modulate the aging process. We found that pathways transcriptionally correlated with diet-modulated lifespan and physiological changes were enriched for lifespan-modifying genes. Male C57BL/6J mice at 4 weeks of age were purchased from Shanghai Animal Co, Ltd. Mice were maintained under a 12-hour dark/light cycle (lights on at 6:30 am) at a temperature of 22 \u00b1 3 \u00b0C in accredited animal facilities. Prior to the start of experiment, mice were maintained on a low-fat diet (Research Diets Inc., New Brunswick, NJ) for one week. At the age of 5 weeks, animals were randomly assigned to one of the 6 intervention groups (n = 30 for each group): feeding of a low-fat diet (10% fat, D12450B, Research Diets) ad libitum (LF) or with 30% calorie restriction (LF+CR) or with voluntary running exercise (LF+Ex), feeding of a high-fat diet (60% fat, D12492, Research Diets) ad libitum (HF) or with 30% calorie restriction (HF+CR) or with voluntary running exercise (HF+Ex). All mice were housed individually during the study. The daily consumption of food in LF and HF groups was recorded over a week and averaged to determine the amount of food for the following week for the LF+CR and HF+CR groups, respectively. After 1 week acclimation in cage with the locked running wheels, mice in the LF+Ex and HF+Ex groups were allowed free access to a running wheel, and the running distance and time were recorded automatically by the equipment. The hepatic transcriptional level for 3 mice from each intervention group at 62 weeks of age was analyzed using Affymetrix Mouse Genome 430 2.0 Arrays.", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-36838", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-36838/samples/"}