{"owner": "ArrayExpress Uploader", "ownerprofile_id": "arrayexpress_sid", "species": "mouse", "factors": [{"GSM9025": {"GENOTYPE": "wild type"}}, {"GSM9025": {"GENOTYPE": "wild type"}}, {"GSM9025": {"GENOTYPE": "wild type"}}, {"GSM9025": {"GENOTYPE": "wild type"}}, {"GSM9025": {"GENOTYPE": "wild type"}}, {"GSM9025": {"GENOTYPE": "wild type"}}, {"GSM902517": {"GENOTYPE": "IL-1R knock-out"}}, {"GSM902517": {"GENOTYPE": "IL-1R knock-out"}}, {"GSM902517": {"GENOTYPE": "IL-1R knock-out"}}, {"GSM902517": {"GENOTYPE": "IL-1R knock-out"}}, {"GSM902517": {"GENOTYPE": "IL-1R knock-out"}}, {"GSM902517": {"GENOTYPE": "IL-1R knock-out"}}], "id": 6831, "pop_total": 0, "platform": 6, "summary_wrapped": "Neutrophil abscess formation is critical in innate immunity against many pathogens. Here, the mechanism of neutrophil abscess formation...", "pubmed_id": 23209417, "geo_gse_id": "E-GEOD-36826", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 1, "sample_count": 12, "tags": ["neutrophil", "skin"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_id_plat": "E-GEOD-36826_A-AFFY-45", "slug": "neutrophil-derived-il-1-is-sufficient-for-abscess", "geo_gds_id": "", "name": "Neutrophil-derived IL-1\u03b2 is sufficient for abscess formation in immunity against Staphylococcus aureus in mice", "created": "Nov.12, 2014", "summary": "Neutrophil abscess formation is critical in innate immunity against many pathogens. Here, the mechanism of neutrophil abscess formation was investigated using a mouse model of Staphylococcus aureus cutaneous infection. Gene expression analysis of S. aureus-infected skin revealed that induction of neutrophil recruitment genes was largely dependent upon IL-1beta/IL-1R activation. Unexpectedly, using IL 1beta reporter mice, neutrophils were identified as the primary source of IL-1beta at the site of infection. Furthermore, IL-1beta-producing neutrophils were necessary and sufficient for abscess formation and bacterial clearance. S. aureus-induced IL 1beta production by neutrophils required TLR2, NOD2, FPRs and the ASC/NLRP3 inflammasome. Taken together, IL-1beta and neutrophil abscess formation during an infection are functionally, spatially and temporally linked as a consequence of direct IL-1beta production by neutrophils. Lesional skin biopsies obtained from C57BL/6J WT mice or IL-1R-deficient mice at 4 hours post-infection with Staphylococcus aureus.  Uninfected skin biopsies were also collected from WT and IL-1R-deficient mice.", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-36826", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-36826/samples/"}