{"owner": "ArrayExpress Uploader", "pop_total": 0, "species": "mouse", "factors": [{"GSM897562": {"VARIATION": "wild-type"}}, {"GSM897562": {"VARIATION": "wild-type"}}, {"GSM897562": {"VARIATION": "wild-type"}}, {"GSM897565": {"VARIATION": "EKLF knock out"}}, {"GSM897565": {"VARIATION": "EKLF knock out"}}, {"GSM897565": {"VARIATION": "EKLF knock out"}}], "id": 6824, "ownerprofile_id": "arrayexpress_sid", "platform": 6, "summary_wrapped": "EKLF is a Kr\u00fcppel-like transcription factor identified as a transcriptional activator and chromatin modifier in erythroid cells. EKLF-...", "geo_gse_id": "E-GEOD-36618", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 1, "sample_count": 6, "tags": ["anemia", "cell", "chromatin", "protein", "proximal"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_id_plat": "E-GEOD-36618_A-AFFY-45", "slug": "mechanisms-of-terminal-erythroid-differentiation-d", "geo_gds_id": "", "name": "Mechanisms of terminal erythroid differentiation defect in EKLF-deficient mice", "created": "Nov.12, 2014", "summary": "EKLF is a Kr\u00fcppel-like transcription factor identified as a transcriptional activator and chromatin modifier in erythroid cells. EKLF-deficient (Eklf -/-) mice die at day 14.5 of gestation from severe anemia. In this study, we demonstrate that early progenitor cells fail to undergo terminal erythroid differention in Eklf -/- embryos. To discover potential EKLF target genes responsible for the failure of erythropoiesis, transcriptional profiling was performed with RNA from wild type and Eklf -/- early erythroid progenitor cells. These analyses identified significant perturbation of a network of genes involved in cell cycle regulation, with the critical regulator of the cell cycle, E2f2, at a hub. E2f2 mRNA and protein levels were markedly decreased in Eklf -/- early erythroid progenitor cells, which showed a delay in the G1-to-S-phase transition. Chromatin immunoprecipitation analysis demonstrated EKLF occupancy at the proximal E2f2 promoter in vivo. Consistent with the role of EKLF as a chromatin modifier, EKLF binding-sites in the E2f2 promoter were located in a region of EKLF-dependent DNase I sensitivity in early erythroid progenitor cells. We propose a model in which EKLF-dependent activation and modification of the E2f2 locus is required for cell cycle progression preceding terminal erythroid differentiation. RNA was isolated from flow-sorted early erythroid progenitors in 13.5 day old fetal livers from EKLF knock out mice (n=3 fetal livers) and wild-type control mice (n=3 fetal livers) for gene expression analysis", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-36618", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-36618/samples/"}