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Home › Dataset Library › Expression data from the adipose tissue of mice fed a normal diet and a high fat diet.

Dataset: Expression data from the adipose tissue of mice fed a normal diet and a high fat diet.

To identify novel Peroxisome Proliferator-Activated Receptor gamma (PPARg) responsive secretory and/or transmembrane genes that is...

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To identify novel Peroxisome Proliferator-Activated Receptor gamma (PPARg) responsive secretory and/or transmembrane genes that is related to obesity, we integrated the expression data from the adipose tissue derived from obese mice with the other two data sets: expression profiling of adipocyte differentiation using ST2 cells and siRNA-mediated knockdown of Pparg during ST2 cell adipogenesis. We used microarrays to detect the up-regulated genes in adipose tissue derived from mice fed a high fat diet compared to a control. Total RNA from adipose tissue was obtained and from mice fed a high fat diet HFD32 (MOUSE_HFD) from 6 week-old to 18 week-old, or a normal diet CE-2 (MOUSE_ND) as a control. Pooled RNAs of each three animals were analyzed by the Affymetrix GeneChip microarray system.

Species:
mouse

Samples:
2

Source:
E-GEOD-36492

Updated:
Dec.12, 2014

Registered:
Nov.12, 2014


Factors: (via ArrayExpress)
Sample DIET
GSM895819 normal diet (CE-2, as a control)
GSM895820 high fat diet (HFD32)

Tags

  • adipocyte
  • adipose tissue
  • cell
  • obesity
  • peroxisome

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