{"owner": "ArrayExpress Uploader", "ownerprofile_id": "arrayexpress_sid", "species": "human", "factors": [{"GSM8944": {"DISEASE STATE": "multiple myeloma", "AGE": "56", "MM STAGE": "IIIa", "SEX": "female"}}, {"GSM894410": {"DISEASE STATE": "multiple myeloma", "AGE": "65", "MM STAGE": "IIIa", "SEX": "male"}}, {"GSM894409": {"DISEASE STATE": "multiple myeloma", "AGE": "47", "MM STAGE": "IIIa", "SEX": "female"}}, {"GSM894408": {"DISEASE STATE": "multiple myeloma", "AGE": "52", "MM STAGE": "IIIb", "SEX": "female"}}, {"GSM894407": {"DISEASE STATE": "healthy", "AGE": "58", "MM STAGE": "not specified", "SEX": "male"}}, {"GSM894406": {"DISEASE STATE": "healthy", "AGE": "45", "MM STAGE": "not specified", "SEX": "female"}}, {"GSM894405": {"DISEASE STATE": "healthy", "AGE": "50", "MM STAGE": "not specified", "SEX": "female"}}], "id": 2500, "pop_total": 0, "platform": 4, "summary_wrapped": "It is now well established that bone marrow (BM) constitutes a microenvironment required for differentiation. Bone marrow mesenchymal...", "geo_gse_id": "E-GEOD-36474", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 4, "sample_count": 7, "tags": ["bone", "bone marrow", "cell", "fibronectin", "genome", "interleukin", "interleukin-6", "multiple myeloma", "myeloma", "syndecan"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_gds_id": "", "slug": "comparison-of-bone-marrow-mesenchymal-stromal-cell", "geo_id_plat": "E-GEOD-36474_A-AFFY-44", "name": "Comparison of bone-marrow mesenchymal stromal cells from multiple myeloma patients and healthy donors", "created": "Jul.12, 2014", "summary": "It is now well established that bone marrow (BM) constitutes a microenvironment required for differentiation. Bone marrow mesenchymal stromal cells (BM-MSCs) strongly support MM cell growth, by producing a high level of Interleukin-6 (IL-6), a major MM cell growth factor. BM-MSCs also support osteoclastogenesis and angiogenesis. Previous studies have suggested that the direct (VLA-4, VCAM-1, CD44, VLA-5, LFA-1, syndecan-1,\u2026) and indirect interactions (soluble factors) between MM plasma cells and BM-MSCs result in constitutive abnormalities in BM-MSCs. In particular, MM BM-MSCs express less CD106 and fibronectin and more DKK1, IL-1\u03b2 and TNF-\u03b1 as compared with normal BM-MSCs.  In order to gain a global view of the differences between BM-MSCs from MM patients and healthy donors, we used gene expression profiling to identify genes associated to the transformation of MM BM-MSCs. BM-MSCs were isolated from 3 healthy donors and 4 untreated multiple myeloma patients.  Total RNA from BM-MSCs was exctracted and hybridyzed on Affymetrix GeneChip\u00ae Human Genome U133 Plus 2.0 Array. Amplification, hybridization and scanning were done according to standard Affymetrix protocols (www.affymetrix.com). CEL files were normalized with RMA method.", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-36474", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-36474/samples/"}