{"owner": "ArrayExpress Uploader", "ownerprofile_id": "arrayexpress_sid", "species": "mouse", "factors": [{"GSM892935": {"CULTURE CONDITION": "mono-cultured (control)"}}, {"GSM892935": {"CULTURE CONDITION": "mono-cultured (control)"}}, {"GSM892937": {"CULTURE CONDITION": "co-cultured for 5 days with leukemic B-cells (CLL V128)"}}, {"GSM892938": {"CULTURE CONDITION": "co-cultured for 5 days with leukemic B-cells (CLL V131)"}}, {"GSM892939": {"CULTURE CONDITION": "co-cultured for 5 days with leukemic B-cells (CLL V190)"}}, {"GSM892940": {"CULTURE CONDITION": "co-cultured for 5 days with leukemic B-cells (CLL V172)"}}, {"GSM89294": {"CULTURE CONDITION": "co-cultured for 5 days with leukemic B-cells (CLL V163)"}}, {"GSM892942": {"CULTURE CONDITION": "co-cultured for 5 days with leukemic B-cells (CLL V211)"}}], "id": 6810, "pop_total": 0, "platform": 6, "summary_wrapped": "Tumor cell survival critically depends on heterotypic communication with benign cells in the microenvironment. Here we describe a novel...", "geo_gse_id": "E-GEOD-36414", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 1, "sample_count": 8, "tags": ["bone", "bone marrow", "breast", "cancer", "cell", "chronic lymphocytic leukemia", "leukemia"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_id_plat": "E-GEOD-36414_A-AFFY-45", "slug": "gene-expression-changes-induced-in-the-stromal-cel", "geo_gds_id": "", "name": "Gene expression changes induced in the stromal cell line EL08-1D2 by co-culture with leukemic B cells (CLL)", "created": "Nov.12, 2014", "summary": "Tumor cell survival critically depends on heterotypic communication with benign cells in the microenvironment. Here we describe a novel survival signaling pathway activated in stromal cells by contact to B-cells from chronic lymphocytic leukemia (CLL) patients.  The expression of PKC-\u03b2II and the subsequent activation of NF-\u03baB in bone marrow stromal cells is a prerequisite to support the survival of malignant B-cells. PKC-\u03b2 knockout mice are insusceptible to CLL-transplantations, underscoring the in vivo significance of the PKC-\u03b2II- NF-\u03baB signaling pathway in the tumor microenvironment. Upregulated stromal PKC-\u03b2II in biopsies from CLL, breast- and pancreatic- cancer patients suggest that this pathway may commonly be activated in a variety of malignancies. We used microarrays to determine gene expression changes induced by co-culturing with leukemic B-cells (CLL) in EL08-1D2 cells. We compared  EL08-1D2 cells co-cultured for 5 days with leukemic B-cells to EL08-1D2 mono-cultured cells by microarray analysis on Affymetrix MG-430_2.0 arrays.", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-36414", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-36414/samples/"}