ArrayExpress Uploader0humandonor 1 (MSC population #276)female42 yrs (middle-aged)passage 1donor 2 (MSC population #353)female67 yrs (middle-aged)passage 2donor 3 (MSC population #295)male61 yrs (middle-aged)passage 1donor 4 (MSC population #296)female62 yrs (middle-aged)passage 1donor 5 (MSC population #247)female56 yrs (middle-aged)passage 1donor 1 (MSC population #276)female42 yrslong term-cultivation (cellular senescence state)donor 2 (MSC population #278)female59 yrslong term-cultivation (cellular senescence state)donor 3 (MSC population #271)female64 yrslong term-cultivation (cellular senescence state)donor 4 (MSC population #277)male54 yrslong term-cultivation (cellular senescence state)donor 5 (MSC population #274)male63 yrslong term-cultivation (cellular senescence state)4425arrayexpress_sid4In the present study we analyzed the effect of cellular senescence on the transcriptome of human mesenchymal stem cells (hMSC;...E-GEOD-35957/profile/8773/arrayexpressuploader410bonebone marrowhiposteoarthritisDec.12, 2014Falseeffects-of-cellular-senescence-on-human-mesenchymaE-GEOD-35957_A-AFFY-44Effects of Cellular Senescence on Human Mesenchymal Stem CellsSep.19, 2014In the present study we analyzed the effect of cellular senescence on the transcriptome of human mesenchymal stem cells (hMSC; alternatively named mesenchymal stromal cells) from bone marrow. Human MSC were isolated from femoral heads of non-osteoporotic donors after total hip arthroplasty. Cells were isolated from human bone marrow according to the previously described protocol (Noth et al., 2002, J Orthop Res, 20/5, 1060-1069) under agreement of the local Ethics Committee of the University of Würzburg. Bone marrow was obtained of femoral heads after total hip arthroplasty due to osteoarthritis and/or hip dysplasia. MSC were replated after reaching 70-90% confluence until they entered state of cellular senescence. RNA samples of control cells were taken from passage 1 or passage 2.http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-35957http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-35957/samples/