ArrayExpress Uploader0humandonor 1 (MSC population #276)female42 yrs (middle-aged)passage 1donor 2 (MSC population #353)female67 yrs (middle-aged)passage 2donor 3 (MSC population #295)male61 yrs (middle-aged)passage 1donor 4 (MSC population #296)female62 yrs (middle-aged)passage 1donor 5 (MSC population #247)female56 yrs (middle-aged)passage 1donor 1 with primary osteoporosis (MSC population #535)female79 yrs (elderly)passage 1donor 2 with primary osteoporosis (MSC population #547)female94 yrs (elderly)passage 1donor 3 with primary osteoporosis (MSC population #558)female87 yrs (elderly)passage 2donor 4 with primary osteoporosis (MSC population #572)female82 yrs (elderly)passage 1donor 5 with primary osteoporosis (MSC population #573)female89 yrs (elderly)passage 14424arrayexpress_sid4In the present study we analyzed the effect of primary osteoporosis and advanced donor age on the transcriptome of human mesenchymal stem...E-GEOD-35956/profile/8773/arrayexpressuploader410bonebone marrowhipmiddleneckosteoarthritisosteoporosisDec.12, 2014Falseeffects-of-primary-osteoporosis-and-advanced-age-oE-GEOD-35956_A-AFFY-44Effects of Primary Osteoporosis and Advanced Age on Human Mesenchymal Stem CellsSep.19, 2014In the present study we analyzed the effect of primary osteoporosis and advanced donor age on the transcriptome of human mesenchymal stem cells (hMSC; alternatively named mesenchymal stromal cells) from bone marrow. Human MSC of elderly patients suffering from osteoporosis were isolated from femoral heads after low-energy fracture of the femoral neck. Control cells were obtained from bone marrow of femoral heads of middle-aged, non-osteoporotic donors after total hip arthroplasty. Cells were isolated from human bone marrow according to the previously described protocol (Noth et al., 2002, J Orthop Res, 20/5, 1060-1069) under agreement of the local Ethics Committee of the University of Würzburg. Human MSC of elderly patients suffering from osteoporosis were isolated from femoral heads after low-energy fracture of the femoral neck. Additional criteria for confirming primary osteoporosis in these donors were vertebrae fractures and advanced age. Bone marrow of middle-aged, non-osteoporotic donors was obtained of femoral heads after total hip arthroplasty due to osteoarthritis and/or hip dysplasia. RNA samples were taken from passage 1 or passage 2.http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-35956http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-35956/samples/