ArrayExpress Uploader0humandonor 1 (MSC population #276)female42 yrs (middle-aged)passage 1donor 2 (MSC population #353)female67 yrs (middle-aged)passage 2donor 3 (MSC population #295)male61 yrs (middle-aged)passage 1donor 4 (MSC population #296)female62 yrs (middle-aged)passage 1donor 5 (MSC population #247)female56 yrs (middle-aged)passage 1donor 1 (MSC population #520)female79 yrs (elderly-aged)passage 1donor 2 (MSC population #559)male79 yrs (elderly-aged)passage 1donor 3 (MSC population #606)female80 yrs (elderly-aged)passage 1donor 4 (MSC population #663)female89 yrs (elderly-aged)passage 14423arrayexpress_sid4In the present study we analyzed the effect of advanced donor age on the transcriptome of human mesenchymal stem cells (hMSC;...E-GEOD-35955/profile/8773/arrayexpressuploader49bonebone marrowhipmiddleosteoarthritisosteoporosisDec.12, 2014Falseeffects-of-aging-on-human-mesenchymal-stem-cellsE-GEOD-35955_A-AFFY-44Effects of aging on Human Mesenchymal Stem CellsSep.19, 2014In the present study we analyzed the effect of advanced donor age on the transcriptome of human mesenchymal stem cells (hMSC; alternatively named mesenchymal stromal cells) from bone marrow. Human MSC of elderly and middle-aged patients without symptoms of osteoporosis were isolated from femoral heads after total hip arthroplasty. Cells were isolated from human bone marrow according to the previously described protocol (Noth et al., 2002, J Orthop Res, 20/5, 1060-1069) under agreement of the local Ethics Committee of the University of Würzburg. Bone marrow was obtained of femoral heads after total hip arthroplasty due to osteoarthritis and/or hip dysplasia. RNA samples were taken from passage 1 or passage 2.http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-35955http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-35955/samples/