ArrayExpress Uploader0humanstimuated with HC1259 plasmastimuated with HC1369 plasmastimuated with HC381 plasmastimuated with HC389 plasmastimuated with HC1534 plasmastimuated with HC1730 plasmastimuated with HC217 plasmastimuated with HC226 plasmastimuated with HC2301 plasmastimuated with HC294 plasmastimuated with HC295 plasmastimuated with HC385 plasmastimuated with HC390 plasmastimuated with HC393 plasmastimuated with HC1006 plasmastimuated with HC272 plasmastimuated with HC285 plasmastimuated with HC233 plasmastimuated with gram-positive pneumonia patient 1353 plasmastimuated with gram-positive pneumonia patient 1382 plasmastimuated with gram-positive pneumonia patient 1403 plasmastimuated with gram-positive pneumonia patient 1410 plasmastimuated with gram-positive pneumonia patient 1412 plasmastimuated with gram-positive pneumonia patient 1224 plasmastimuated with gram-positive pneumonia patient 1275 plasmastimuated with gram-positive pneumonia patient 1287 plasmastimuated with gram-positive pneumonia patient 1325 plasmastimuated with gram-positive pneumonia patient 1344 plasma4417arrayexpress_sid4The complex milieu of inflammatory mediators associated with many diseases is often too dilute to directly measure in the periphery,...E-GEOD-35716/profile/8773/arrayexpressuploader128bacterial pneumoniaperipheralpneumoniaDec.12, 2014Falsetranscriptional-signatures-as-a-disease-specific-3E-GEOD-35716_A-AFFY-44Transcriptional Signatures as a Disease-Specific and Predictive Inflammatory Biomarker for Type 1 Diabetes [Pneu_S3S24_10Pneu_4HC]Sep.19, 2014The complex milieu of inflammatory mediators associated with many diseases is often too dilute to directly measure in the periphery, necessitating development of more sensitive measurements suitable for mechanistic studies, earlier diagnosis, guiding selection of therapy, and monitoring interventions. Previously, we determined that plasma of recent-onset (RO) Type 1 diabetes (T1D) patients induce a proinflammatory transcriptional signature in fresh peripheral blood mononuclear cells (PBMC) relative to that of unrelated healthy controls (HC). Here, using an optimized cryopreserved PBMC-based protocol, we compared the signature found between unrelated healthy controls and patients with bacterial pneumonia. UPN727 cells were stimulated with either plasma from unrelated healthy controls (n=18) or plasma from patients with bacterial pneumonia (n=10). Gene expression analysis was perfromed in order to evaluate the transcriptional signature associated with bacterial pneumonia.http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-35716http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-35716/samples/