{"owner": "ArrayExpress Uploader", "pop_total": 0, "species": "human", "factors": [{"GSM873478": {"CELL TYPE": "human bone marrow CD38-"}}, {"GSM873478": {"CELL TYPE": "human bone marrow CD38-"}}, {"GSM873478": {"CELL TYPE": "human bone marrow CD38-"}}, {"GSM87348": {"CELL TYPE": "human bone marrow CD62Lhi"}}, {"GSM87348": {"CELL TYPE": "human bone marrow CD62Lhi"}}, {"GSM87348": {"CELL TYPE": "human bone marrow CD62Lhi"}}, {"GSM873484": {"CELL TYPE": "human bone marrow CD10+"}}, {"GSM873484": {"CELL TYPE": "human bone marrow CD10+"}}, {"GSM873484": {"CELL TYPE": "human bone marrow CD10+"}}], "id": 4413, "ownerprofile_id": "arrayexpress_sid", "platform": 4, "summary_wrapped": "Studies of adult human hematopoiesis have until now relied on the expression of CD10 to define lymphoid commitment. We report a novel...", "geo_gse_id": "E-GEOD-35685", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 1, "sample_count": 9, "tags": ["bone", "bone marrow", "cell", "genome", "intermediate", "l-selectin", "selectin"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_gds_id": "", "slug": "lymphoid-priming-in-human-bone-marrow-begins-prior", "geo_id_plat": "E-GEOD-35685_A-AFFY-44", "name": "Lymphoid Priming in Human Bone Marrow Begins Prior to CD10 Expression with Up-Regulation of L-selectin", "created": "Sep.19, 2014", "summary": "Studies of adult human hematopoiesis have until now relied on the expression of CD10 to define lymphoid commitment. We report a novel lymphoid-primed population in human bone marrow that is generated from hematopoietic stem cells (HSC) prior to the onset of CD10 expression and B cell commitment, and is identified by high levels of the homing molecule L-selectin (CD62L). CD10-CD62Lhi progenitors have full lymphoid (B/T/NK) potential, and show reduced myeloid and absent erythroid potential. Genome-wide gene expression analysis demonstrates that the CD10-CD62Lhi population represents an intermediate stage of differentiation between CD34+CD38- HSC and CD34+lin-CD10+ progenitors marked by down-regulation of TAL1 and MPL, upregulation of E2A, CD3E and IL2RG expression, and absent B cell commitment or RAG1/2 expression. Immature CD34+CD1a- thymocytes are also CD62Lhi and L-selectin ligands are expressed at the cortico-medullary junction, suggesting a possible role for L-selectin in human thymic homing. These studies identify the earliest stage of lymphoid priming in human bone marrow. Freshly harvested human bone marrow was ficoll purified, enriched for CD34+, and then FACS sorted. It was then sorted into 3 samples CD34+CD38-, CD34+ CD10- CD62L++, and CD34+ CD10+, with 3 independent biological replicates", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-35685", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-35685/samples/"}