{"owner": "ArrayExpress Uploader", "pop_total": 0, "id": 6772, "factors": [{"GSE3554GSM81691": {"clinical information": "normal"}}, {"GSE3554GSM81691": {"clinical information": "normal"}}, {"GSE3554GSM81691": {"clinical information": "normal"}}, {"GSE3554GSM81688": {"clinical information": "elevated intraocular pressure"}}, {"GSE3554GSM81688": {"clinical information": "elevated intraocular pressure"}}, {"GSE3554GSM81688": {"clinical information": "elevated intraocular pressure"}}], "ownerprofile_id": "arrayexpress_sid", "platform": 6, "summary_wrapped": "Purpose: The DBA/2J mouse is a model for secondary angle-closure glaucoma due to iris atrophy and pigment dispersion, which ultimately...", "pubmed_id": 16505032, "geo_gse_id": "E-GEOD-3554", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 1, "sample_count": 6, "tags": ["angle-closure glaucoma", "disease", "glaucoma", "iris", "retina"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_gds_id": "", "slug": "transcription-profiling-by-array-of-dba2j-mouse-mo", "geo_id_plat": "E-GEOD-3554_A-AFFY-45", "name": "Transcription profiling by array of DBA/2J mouse model of glaucoma with elevated intraocular pressure", "created": "Nov.12, 2014", "summary": "Purpose: The DBA/2J mouse is a model for secondary angle-closure glaucoma due to iris atrophy and pigment dispersion, which ultimately leads to increased intraocular pressure (IOP). We sought to correlate changes in retinal gene expression with glaucoma-like pathology by performing microarray analysis of retinal RNA from DBA/2J mice at 3 months before disease onset, and at 8 months, after IOP elevation. Methods: IOP was monitored monthly in DBA/2J animals by Tono-Pen and animals with normal (3 months) or elevated IOP (8 months) were identified. RNA was prepared from 3 individual retinas at each age, and the RNA was amplified and used to generate biotin-labeled probe for high density mouse Affymetrix arrays (U430.2). A subset of genes was selected for confirmation by quantitative RT-PCR using independent retina samples from DBA/2J animals at 3, 5 and 8 months of age, and compared to retinas from C57BL/6J control animals at 3 and 8 months. Results: There were changes in expression of 68 genes, with 32 genes increasing and 36 genes decreasing at 8 months versus 3 months. Upregulated genes were associated with immune response, glial activation, signaling and gene expression, while down-regulated genes included multiple crystallin genes. Significant changes in 9 upregulated genes and 2 downregulated genes were confirmed by quantitative RT-PCR, with some showing changes in expression by 5 months. Conclusions: DBA/2J retina shows evidence for glial activation and an immune-related response following IOP elevation, similar to what has been reported following acute elevation of IOP in other models.  IOP was monitored monthly in DBA/2J animals by Tono-Pen and animals with normal (3 months) or elevated IOP (8 months) were identified. RNA was prepared from 3 individual retinas at each age, and the RNA was amplified and used to generate biotin-labeled probe for high density mouse Affymetrix arrays (U430.2). A subset of genes was selected for confirmation by quantitative RT-PCR using independent retina samples from DBA/2J animals at 3, 5 and 8 months of age, and compared to retinas from C57BL/6J control animals at 3 and 8 months.", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-3554", "species": "mouse", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-3554/samples/"}