ArrayExpress Uploaderarrayexpress_sidhumanpcDNA6/V5-His plasmidpcDNA6/V5-His-ZNF217 plasmidpcDNA6/V5-His plasmidpcDNA6/V5-His-ZNF217 plasmid440304To obtain an overview of the cellular functions regulated by ZNF217 signaling in breast-cancer cell lines, we performed global gene-...E-GEOD-35511/profile/8773/arrayexpressuploader14breastcancercellgenomeDec.12, 2014FalseE-GEOD-35511_A-AFFY-44gene-expression-profiling-of-znf217-overexpressingGene-expression profiling of ZNF217-overexpressing MDA-MB-231 cellsSep.19, 2014To obtain an overview of the cellular functions regulated by ZNF217 signaling in breast-cancer cell lines, we performed global gene-expression profiling on MDA-MB-231-pcDNA6 and MDA-MB-231-ZNF217 cells Two independent cell-culture replicates from the total population of MDA-MB-231-pcDNA6 and MDA-MB-231-ZNF217 stable transfectants were used to generate total RNA. Total RNA was extracted from cell culture using a Qiagen RNA extraction kit and RNA quality was assessed using the BioAnalyzer 2100™ (Agilent Technologies). Complex probes were produced from these RNA, then hybridized to Human genome U133 Plus 2.0 array according to the manufacturer’s recommendations (Affymetrix).http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-35511http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-35511/samples/