{"owner": "ArrayExpress Uploader", "ownerprofile_id": "arrayexpress_sid", "species": "human", "factors": [{"GSM870010": {"TRANSFECTION": "pcDNA6/V5-His plasmid"}}, {"GSM8700": {"TRANSFECTION": "pcDNA6/V5-His-ZNF217 plasmid"}}, {"GSM870010": {"TRANSFECTION": "pcDNA6/V5-His plasmid"}}, {"GSM8700": {"TRANSFECTION": "pcDNA6/V5-His-ZNF217 plasmid"}}], "id": 4403, "pop_total": 0, "platform": 4, "summary_wrapped": "To obtain an overview of the cellular functions regulated by ZNF217 signaling in breast-cancer cell lines, we performed global gene-...", "geo_gse_id": "E-GEOD-35511", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 1, "sample_count": 4, "tags": ["breast", "cancer", "cell", "genome"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_id_plat": "E-GEOD-35511_A-AFFY-44", "slug": "gene-expression-profiling-of-znf217-overexpressing", "geo_gds_id": "", "name": "Gene-expression profiling of ZNF217-overexpressing MDA-MB-231 cells", "created": "Sep.19, 2014", "summary": "To obtain an overview of the cellular functions regulated by ZNF217 signaling in breast-cancer cell lines, we performed global gene-expression profiling on MDA-MB-231-pcDNA6 and MDA-MB-231-ZNF217 cells Two independent cell-culture replicates from the total population of MDA-MB-231-pcDNA6 and MDA-MB-231-ZNF217 stable transfectants were used to generate total RNA. Total RNA was extracted from cell culture using a Qiagen RNA extraction kit and RNA quality was assessed using the BioAnalyzer 2100\u2122 (Agilent Technologies). Complex probes were produced from these RNA, then hybridized to Human genome U133 Plus 2.0 array according to the manufacturer\u2019s recommendations (Affymetrix).", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-35511", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-35511/samples/"}