Dataset: Gene-expression profiling of ZNF217-overexpressing MDA-MB-231 cells
To obtain an overview of the cellular functions regulated by ZNF217 signaling in breast-cancer cell lines, we performed global gene-...
To obtain an overview of the cellular functions regulated by ZNF217 signaling in breast-cancer cell lines, we performed global gene-expression profiling on MDA-MB-231-pcDNA6 and MDA-MB-231-ZNF217 cells Two independent cell-culture replicates from the total population of MDA-MB-231-pcDNA6 and MDA-MB-231-ZNF217 stable transfectants were used to generate total RNA. Total RNA was extracted from cell culture using a Qiagen RNA extraction kit and RNA quality was assessed using the BioAnalyzer 2100™ (Agilent Technologies). Complex probes were produced from these RNA, then hybridized to Human genome U133 Plus 2.0 array according to the manufacturer’s recommendations (Affymetrix).
- Species:
- human
- Samples:
- 4
- Source:
- E-GEOD-35511
- Updated:
- Dec.12, 2014
- Registered:
- Sep.19, 2014
Sample | TRANSFECTION |
---|---|
GSM870010 | pcDNA6/V5-His plasmid |
GSM8700 | pcDNA6/V5-His-ZNF217 plasmid |
GSM870010 | pcDNA6/V5-His plasmid |
GSM8700 | pcDNA6/V5-His-ZNF217 plasmid |