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Dataset: Gene-expression profiling of ZNF217-overexpressing MDA-MB-231 cells

To obtain an overview of the cellular functions regulated by ZNF217 signaling in breast-cancer cell lines, we performed global gene-...

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To obtain an overview of the cellular functions regulated by ZNF217 signaling in breast-cancer cell lines, we performed global gene-expression profiling on MDA-MB-231-pcDNA6 and MDA-MB-231-ZNF217 cells Two independent cell-culture replicates from the total population of MDA-MB-231-pcDNA6 and MDA-MB-231-ZNF217 stable transfectants were used to generate total RNA. Total RNA was extracted from cell culture using a Qiagen RNA extraction kit and RNA quality was assessed using the BioAnalyzer 2100™ (Agilent Technologies). Complex probes were produced from these RNA, then hybridized to Human genome U133 Plus 2.0 array according to the manufacturer’s recommendations (Affymetrix).

Species:
human

Samples:
4

Source:
E-GEOD-35511

Updated:
Dec.12, 2014

Registered:
Sep.19, 2014


Factors: (via ArrayExpress)
Sample TRANSFECTION
GSM870010 pcDNA6/V5-His plasmid
GSM8700 pcDNA6/V5-His-ZNF217 plasmid
GSM870010 pcDNA6/V5-His plasmid
GSM8700 pcDNA6/V5-His-ZNF217 plasmid

Tags

  • breast
  • cancer
  • cell
  • genome

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