ArrayExpress Uploader0mouse1thioglycolate-elicited murine peritoneal macrophagesincubated in OptiMEM-10 for 24 hrs on Permanox plastic2thioglycolate-elicited murine peritoneal macrophagesincubated in OptiMEM-10 + IL-4 (supernatant from IL-4 producing cell line) for 24 hrs on Permanox plastic3thioglycolate-elicited murine peritoneal macrophagesincubated in OptiMEM-10 for 24 hrs on tissue culture plastic1thioglycolate-elicited murine peritoneal macrophagesincubated in OptiMEM-10 + IL-4 (supernatant from IL-4 producing cell line) for 24 hrs on tissue culture plastic2biogel-elicited murine peritoneal macrophagesincubated in OptiMEM-10 for 24 hrs on tissue culture plastic3biogel-elicited murine peritoneal macrophagesincubated in OptiMEM-10 + IL-4 (supernatant from IL-4 producing cell line) for 24 hrs on tissue culture plastic6770arrayexpress_sid6Analysis of alternative activation of macrophages at gene expression level. The study forms part of a wider study where we compare the...E-GEOD-35436/profile/8773/arrayexpressuploader36cytokinemacrophageDec.12, 2014Falsegenome-wide-analysis-of-mouse-macrophages-stimul-2E-GEOD-35436_A-AFFY-45Genome-wide analysis of mouse macrophages stimulated with IL-4 (Biogel and thioglycollate macrophages) (Affymetrix)Nov.12, 2014Analysis of alternative activation of macrophages at gene expression level. The study forms part of a wider study where we compare the effects of IL-4 in different human and mouse macrophages. Our results support the notion that in vitro culture conditions greatly affect the macrophage response to IL-4. Total RNA obtained from Thioglycollate and Biogel elicited peritoneal macrophages exposed to 20 ng/ml of IL-4 for 18 hours. Biogel and thioglycollate elicited macrophages were stimulated with the Th2 cytokine IL-4, for RNA extraction and hybridization on Affymetrix microarrays.http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-35436http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-35436/samples/