{"owner": "ArrayExpress Uploader", "pop_total": 0, "species": "mouse", "factors": [{"GSM868299": {"REPLICATE": 1, "CELL TYPE": "thioglycolate-elicited murine peritoneal macrophages", "TREATMENT": "incubated in OptiMEM-10 for 24 hrs on Permanox plastic"}}, {"GSM868300": {"REPLICATE": 2, "CELL TYPE": "thioglycolate-elicited murine peritoneal macrophages", "TREATMENT": "incubated in OptiMEM-10 + IL-4 (supernatant from IL-4 producing cell line) for 24 hrs on Permanox plastic"}}, {"GSM86830": {"REPLICATE": 3, "CELL TYPE": "thioglycolate-elicited murine peritoneal macrophages", "TREATMENT": "incubated in OptiMEM-10 for 24 hrs on tissue culture plastic"}}, {"GSM868302": {"REPLICATE": 1, "CELL TYPE": "thioglycolate-elicited murine peritoneal macrophages", "TREATMENT": "incubated in OptiMEM-10 + IL-4 (supernatant from IL-4 producing cell line) for 24 hrs on tissue culture plastic"}}, {"GSM868303": {"REPLICATE": 2, "CELL TYPE": "biogel-elicited murine peritoneal macrophages", "TREATMENT": "incubated in OptiMEM-10 for 24 hrs on tissue culture plastic"}}, {"GSM868304": {"REPLICATE": 3, "CELL TYPE": "biogel-elicited murine peritoneal macrophages", "TREATMENT": "incubated in OptiMEM-10 + IL-4 (supernatant from IL-4 producing cell line) for 24 hrs on tissue culture plastic"}}], "id": 6770, "ownerprofile_id": "arrayexpress_sid", "platform": 6, "summary_wrapped": "Analysis of alternative activation of macrophages at gene expression level. The study forms part of a wider study where we compare the...", "geo_gse_id": "E-GEOD-35436", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 3, "sample_count": 6, "tags": ["cytokine", "macrophage"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_gds_id": "", "slug": "genome-wide-analysis-of-mouse-macrophages-stimul-2", "geo_id_plat": "E-GEOD-35436_A-AFFY-45", "name": "Genome-wide analysis of mouse macrophages stimulated with IL-4 (Biogel and thioglycollate macrophages) (Affymetrix)", "created": "Nov.12, 2014", "summary": "Analysis of alternative activation of macrophages at gene expression level. The study forms part of a wider study where we compare the effects of IL-4 in different human and mouse macrophages. Our results support the notion that in vitro culture conditions greatly affect the macrophage response to IL-4. Total RNA obtained from Thioglycollate and Biogel elicited peritoneal macrophages exposed to 20 ng/ml of IL-4 for 18 hours. Biogel and thioglycollate elicited macrophages were stimulated with the Th2 cytokine IL-4, for RNA extraction and hybridization on Affymetrix microarrays.", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-35436", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-35436/samples/"}