Dataset: Genome-wide analysis of mouse macrophages stimulated with IL-4 (Biogel and thioglycollate macrophages) (Affymetrix)
Analysis of alternative activation of macrophages at gene expression level. The study forms part of a wider study where we compare the...
Analysis of alternative activation of macrophages at gene expression level. The study forms part of a wider study where we compare the effects of IL-4 in different human and mouse macrophages. Our results support the notion that in vitro culture conditions greatly affect the macrophage response to IL-4. Total RNA obtained from Thioglycollate and Biogel elicited peritoneal macrophages exposed to 20 ng/ml of IL-4 for 18 hours. Biogel and thioglycollate elicited macrophages were stimulated with the Th2 cytokine IL-4, for RNA extraction and hybridization on Affymetrix microarrays.
- Species:
- mouse
- Samples:
- 6
- Source:
- E-GEOD-35436
- Updated:
- Dec.12, 2014
- Registered:
- Nov.12, 2014
Factors:
(via ArrayExpress)
| Sample | REPLICATE | CELL TYPE | TREATMENT |
|---|---|---|---|
| GSM868299 | 1 | thioglycolate-elicited murine peritoneal macrophages | incubated in OptiMEM-10 for 24 hrs on Permanox plastic |
| GSM868300 | 2 | thioglycolate-elicited murine peritoneal macrophages | incubated in OptiMEM-10 + IL-4 (supernatant from IL-4 producing cell line) for 24 hrs on Permanox plastic |
| GSM86830 | 3 | thioglycolate-elicited murine peritoneal macrophages | incubated in OptiMEM-10 for 24 hrs on tissue culture plastic |
| GSM868302 | 1 | thioglycolate-elicited murine peritoneal macrophages | incubated in OptiMEM-10 + IL-4 (supernatant from IL-4 producing cell line) for 24 hrs on tissue culture plastic |
| GSM868303 | 2 | biogel-elicited murine peritoneal macrophages | incubated in OptiMEM-10 for 24 hrs on tissue culture plastic |
| GSM868304 | 3 | biogel-elicited murine peritoneal macrophages | incubated in OptiMEM-10 + IL-4 (supernatant from IL-4 producing cell line) for 24 hrs on tissue culture plastic |