ArrayExpress Uploader06768UTX conditional knockoutUTX conditional knockoutUTX knockoutUTX knockoutarrayexpress_sid6UTX gene is localized on the X chromosome, identified as a demethylase on histone H3 lysine 27. Two independent UTX conditional knockout...22949634E-GEOD-35415/profile/8773/arrayexpressuploader14cellchromosomedishembryonic stem cellgenomehistonehistone h3lysinestem cellx chromosomeDec.12, 2014Falseutx-regulated-genes-in-mouse-embryonic-stem-cellsE-GEOD-35415_A-AFFY-45UTX regulated genes in mouse embryonic stem cellsNov.12, 2014UTX gene is localized on the X chromosome, identified as a demethylase on histone H3 lysine 27. Two independent UTX conditional knockout (cKO) embryonic stem cell lines and two UTX knockout (KO) cell lines were cultured on 0.1% gelatin-coated dishes without feeder cells at desity of 1 million cells/10 cm dish. Cells were cultured overnight and collected. Total RNAs were sequentially purified with Trizol (Invitrogen) and RNeasy kit (Qiagen) and analyzed on Mouse Genome 430 2.0 Array (Affymetrix) at NIDDK Microarray Core Facility following standard protocols.http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-35415mousehttp://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-35415/samples/