ArrayExpress Uploader04395Skinepidermal Langerhans cellsorted CD1a+, epidermal Langerhans cellsSkinepidermal Langerhans cellCD1a+, epidermal Langerhans cellsSkinepidermal Langerhans cellCD1a+, epidermal Langerhans cellsbonenot specifiedsorted CD207+, CD1a+ LCH cellsbonenot specifiedsorted CD207+, CD1a+ LCH cellsbonenot specifiedsorted CD207+, CD1a+ LCH cellsbonenot specifiedsorted CD207+, CD1a+ LCH cellsbonenot specifiedsorted CD207+, CD1a+ LCH cellsSkinnot specifiedsorted CD207+, CD1a+ LCH cellsbonenot specifiedsorted CD207+, CD1a+ LCH cellsmucosanot specifiedsorted CD207+, CD1a+ LCH cellsPeripheral Bloodmyeloid dendritic cells (mDC1)sorted HLA-DR+/CD45RA hi/CD11c-/BDCA2+ cellsPeripheral Bloodmyeloid dendritic cells (mDC1)sorted HLA-DR+/CD45RA hi/CD11c-/BDCA2+ cellsPeripheral Bloodmyeloid dendritic cells (mDC1)sorted HLA-DR+/CD45RA hi/CD11c-/BDCA2+ cellsPeripheral Bloodplasmacytoid dendritic cells (pDC)sorted HLA-DR+/CD11c+/BDCA1+/BDCA3- cellsPeripheral Bloodplasmacytoid dendritic cells (pDC)sorted HLA-DR+/CD11c+/BDCA1+/BDCA3- cellsPeripheral Bloodplasmacytoid dendritic cells (pDC)sorted HLA-DR+/CD11c+/BDCA1+/BDCA3- cellsarrayexpress_sid4Langerhans cell histiocytosis (LCH) is a disease characterized by the accumulation of eponymous CD1a+ Langerin+ Langerhans-cell (LC)-like...23074278E-GEOD-35340/profile/8773/arrayexpressuploader317celldendriticdiseasehistiocytosislangerhans cellperipheralskinDec.12, 2014Falsenotch-is-active-in-langerhans-cell-histiocytosis-aE-GEOD-35340_A-AFFY-44Notch is active in Langerhans Cell Histiocytosis and confers pathognomonic features on dendritic cells.Sep.19, 2014Langerhans cell histiocytosis (LCH) is a disease characterized by the accumulation of eponymous CD1a+ Langerin+ Langerhans-cell (LC)-like dendritic cells (DC) of largely unknown origin. Here we have performed comparative transcriptome analysis of highly purified CD207+/CD1a+ Langerhans cell histiocytosis (LCH) cells derived from different locations and disease courses and three major human dendritic cell lineages: epidermal Langerhans cells, myeloid dendritic cells (mDC1) and plasmacytoid dendritic cells (pDC) in order to investigate the relationship between LCH cells and naturally occurring dendritic cells. Data obtained indicate that LCH cells form a distinct DC entity. Furthermore, we have identified transcripts that are uniquely expressed by LCH cells in comparison to LC, mDC1, and pDC, and induce LCH-specific features in human DC. Primary cells were isolated from peripheral blood (mDC1 and pDC), skin (epidermal Langerhans cells) and CD207+/CD1a+ Langerhans cell histiocytosis (LCH) cells derived from different locations. RNA was isolated from these cells ex vivo.http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-35340humanhttp://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-35340/samples/