Dataset: Expression data from Bone-Marrow Mesenchymal Stromal Cells (BM-MSC) from Follicular Lymphoma patient (FL) or Healthy Donor (HD).
Human bone marrow mesenchymal stromal cells (BM-MSC) could be committed toward a functional lymphoid-like stroma by a combination of...
Human bone marrow mesenchymal stromal cells (BM-MSC) could be committed toward a functional lymphoid-like stroma by a combination of TNFalpha (TNF) and Lymphotoxin alpha1/beta2 (LT) (Amé-Thomas et al Blood 2007). Bone marrow and lymph node stromal cells support FL malignant cell recruitment and growth in particular after comittment to a lymphoid-like differentiation in vitro. In addition, more than 70% of FL patients exhibit a bone marrow involvment at diagnosis. We delineate using Affymetrix U133+2.0 microarrays the gene expression profile of BM-MSC obtained from FL patients (FL-MSC) and age-matched healthy donors (HD-MSC) in order to identify a specific FL-MSC signature. In addition, we used Affymetrix microarrays to define the gene expression signature of lymphoid-like stromal cells obtained from HD-MSC by treatment with TNF/LT in vitro. This TNF/LT signature was then used to interpret the gene expression profile of FL-MSC. GEP was performed on 10 BM-MSC from FL patients and 6 from healthy donors, treated or not with TNF(10 ng/mL)/LT(100ng/mL)
- Species:
- human
- Samples:
- 28
- Source:
- E-GEOD-35331
- Updated:
- Dec.12, 2014
- Registered:
- Sep.19, 2014
| Sample | AGE (Y) | GENDER | PATHOLOGY | GROWTH PROTOCOL | TREATMENT |
|---|---|---|---|---|---|
| GSM866168 | 55 | male | Healthy donor | alphaMEM+10%FBS+FGF2(1ng/mL) | Untreated |
| GSM866169 | 53 | male | Healthy donor | alphaMEM+10%FBS+FGF2(1ng/mL) | Untreated |
| GSM866170 | 30 | male | Healthy donor | alphaMEM+10%FBS+FGF2(1ng/mL) | Untreated |
| GSM86617 | 68 | female | Healthy donor | alphaMEM+10%FBS+FGF2(1ng/mL) | Untreated |
| GSM866169 | 53 | male | Healthy donor | alphaMEM+10%FBS+FGF2(1ng/mL) | Untreated |
| GSM866173 | 78 | female | Healthy donor | alphaMEM+10%FBS+FGF2(1ng/mL) | Untreated |
| GSM866174 | 68 | male | Follicular Lymphoma patient | alphaMEM+10%FBS+FGF2(1ng/mL) | Untreated |
| GSM866175 | 55 | male | Follicular Lymphoma patient | alphaMEM+10%FBS+FGF2(1ng/mL) | Untreated |
| GSM866176 | 76 | male | Follicular Lymphoma patient | alphaMEM+10%FBS+FGF2(1ng/mL) | Untreated |
| GSM866177 | 53 | female | Follicular Lymphoma patient | alphaMEM+10%FBS+FGF2(1ng/mL) | Untreated |
| GSM866178 | 73 | male | Follicular Lymphoma patient | alphaMEM+10%FBS+FGF2(1ng/mL) | Untreated |
| GSM866179 | 61 | male | Follicular Lymphoma patient | alphaMEM+10%FBS+FGF2(1ng/mL) | Untreated |
| GSM866180 | 47 | male | Follicular Lymphoma patient | alphaMEM+10%FBS+FGF2(1ng/mL) | Untreated |
| GSM86618 | 56 | male | Follicular Lymphoma patient | alphaMEM+10%FBS+FGF2(1ng/mL) | Untreated |
| GSM866182 | 42 | male | Follicular Lymphoma patient | alphaMEM+10%FBS+FGF2(1ng/mL) | Untreated |
| GSM866183 | 66 | female | Follicular Lymphoma patient | alphaMEM+10%FBS+FGF2(1ng/mL) | Untreated |
| GSM866168 | 55 | male | Healthy donor | alphaMEM+10%FBS+FGF2(1ng/mL) | Untreated |
| GSM866169 | 53 | male | Healthy donor | alphaMEM+10%FBS+FGF2(1ng/mL) | Untreated |
| GSM866170 | 30 | male | Healthy donor | alphaMEM+10%FBS+FGF2(1ng/mL) | Untreated |
| GSM86617 | 68 | female | Healthy donor | alphaMEM+10%FBS+FGF2(1ng/mL) | Untreated |
| GSM866169 | 53 | male | Healthy donor | alphaMEM+10%FBS+FGF2(1ng/mL) | Untreated |
| GSM866173 | 78 | female | Healthy donor | alphaMEM+10%FBS+FGF2(1ng/mL) | Untreated |
| GSM866190 | 55 | male | Healthy donor | RPMI1640+10%FBS | Stimulated by TNF+LT for 3 days |
| GSM86619 | 53 | male | Healthy donor | RPMI1640+10%FBS | Stimulated by TNF+LT for 3 days |
| GSM866192 | 30 | male | Healthy donor | RPMI1640+10%FBS | Stimulated by TNF+LT for 3 days |
| GSM866193 | 68 | female | Healthy donor | RPMI1640+10%FBS | Stimulated by TNF+LT for 3 days |
| GSM86619 | 53 | male | Healthy donor | RPMI1640+10%FBS | Stimulated by TNF+LT for 3 days |
| GSM866195 | 78 | female | Healthy donor | RPMI1640+10%FBS | Stimulated by TNF+LT for 3 days |