6ArrayExpress Uploaderarrayexpress_sidmouseAmhr2(+/+)-SmoM2 controlAmhr2(cre/+)-SmoM2 mutant67540http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-35123The objective of the study was to determine the cause of anovulation in transgenic mice with conditional over-activation of the hedgehog...22402963/profile/8773/arrayexpressuploader12anovulationovaryDec.12, 2014Falsegene-expression-in-mice-with-conditional-over-actiE-GEOD-35123_A-AFFY-45Gene expression in mice with conditional over-activation of hedgehog signaling in the ovaryNov.12, 2014The objective of the study was to determine the cause of anovulation in transgenic mice with conditional over-activation of the hedgehog signaling pathway in the ovary. In Amhr2(cre/+)-SmoM2 transgenic mice, a constitutively active version of the hedgehog signal transducer, smoothened (SMO), known as SMOM2, is expressed in the ovary following cre-mediated recombination. The hypothesis tested is that changes in the neonatal ovary caused by over-activation of hedgehog signaling leads to the life-long anovulation phenotype in transgenic mice. Microarrays were used to examine differences in gene expression in ovaries of two day old wild-type mice (Amhr2(+/+)-SmoM2) and transgenic mice (Amhr2(cre/+)-SmoM2) in which the hedgehog signaling pathway was conditionally over-activated in the ovary. Two microarrays were performed. RNA samples were prepared from ovaries of 8 Amhr2(+/+)-SmoM2 control mice and 8 Amhr2(cre/+)-SmoM2 mutant mice on day two of age. RNA quality was assessed by measurement of ribosomal RNA on an Agilent 2100 Bioanalyzer.E-GEOD-35123http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-35123/samples/