{"platform": 6, "owner": "ArrayExpress Uploader", "ownerprofile_id": "arrayexpress_sid", "species": "mouse", "factors": [{"GSM86230": {"VARIATION": "Amhr2(+/+)-SmoM2 control"}}, {"GSM862302": {"VARIATION": "Amhr2(cre/+)-SmoM2 mutant"}}], "id": 6754, "pop_total": 0, "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-35123", "summary_wrapped": "The objective of the study was to determine the cause of anovulation in transgenic mice with conditional over-activation of the hedgehog...", "pubmed_id": 22402963, "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 1, "sample_count": 2, "tags": ["anovulation", "ovary"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_gds_id": "", "slug": "gene-expression-in-mice-with-conditional-over-acti", "geo_id_plat": "E-GEOD-35123_A-AFFY-45", "name": "Gene expression in mice with conditional over-activation of hedgehog signaling in the ovary", "created": "Nov.12, 2014", "summary": "The objective of the study was to determine the cause of anovulation in transgenic mice with conditional over-activation of the hedgehog signaling pathway in the ovary.  In Amhr2(cre/+)-SmoM2 transgenic mice, a constitutively active version of the hedgehog signal transducer, smoothened (SMO), known as SMOM2, is expressed in the ovary following cre-mediated recombination. The hypothesis tested is that changes in the neonatal ovary caused by over-activation of hedgehog signaling leads to the life-long anovulation phenotype in transgenic mice. Microarrays were used to examine differences in gene expression in ovaries of two day old wild-type mice (Amhr2(+/+)-SmoM2) and transgenic mice (Amhr2(cre/+)-SmoM2) in which the hedgehog signaling pathway was conditionally over-activated in the ovary. Two microarrays were performed.  RNA samples were prepared from ovaries of 8 Amhr2(+/+)-SmoM2 control mice and 8 Amhr2(cre/+)-SmoM2 mutant mice on day two of age.  RNA quality was assessed by measurement of ribosomal RNA on an Agilent 2100 Bioanalyzer.", "geo_gse_id": "E-GEOD-35123", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-35123/samples/"}