{"owner": "ArrayExpress Uploader", "pop_total": 0, "id": 2625, "factors": [{"GSM860206": {"SOURCE TISSUE": "human ductal breast cancer", "CELL LINE": "BT-474", "TREATMENT": "32 C", "CONDITION": "p53 status on"}}, {"GSM860205": {"SOURCE TISSUE": "human ductal breast cancer", "CELL LINE": "BT-474", "TREATMENT": "37 C", "CONDITION": "p53 status off"}}, {"GSM860206": {"SOURCE TISSUE": "human ductal breast cancer", "CELL LINE": "BT-474", "TREATMENT": "32 C", "CONDITION": "p53 status on"}}, {"GSM860205": {"SOURCE TISSUE": "human ductal breast cancer", "CELL LINE": "BT-474", "TREATMENT": "37 C", "CONDITION": "p53 status off"}}, {"GSM860206": {"SOURCE TISSUE": "human ductal breast cancer", "CELL LINE": "BT-474", "TREATMENT": "32 C", "CONDITION": "p53 status on"}}, {"GSM860205": {"SOURCE TISSUE": "human ductal breast cancer", "CELL LINE": "BT-474", "TREATMENT": "37 C", "CONDITION": "p53 status off"}}, {"GSM860200": {"SOURCE TISSUE": "human ductal breast cancer", "CELL LINE": "MCF-7", "TREATMENT": "32 C", "CONDITION": "p53 status on"}}, {"GSM860199": {"SOURCE TISSUE": "human ductal breast cancer", "CELL LINE": "MCF-7", "TREATMENT": "37 C", "CONDITION": "p53 status on"}}, {"GSM860200": {"SOURCE TISSUE": "human ductal breast cancer", "CELL LINE": "MCF-7", "TREATMENT": "32 C", "CONDITION": "p53 status on"}}, {"GSM860199": {"SOURCE TISSUE": "human ductal breast cancer", "CELL LINE": "MCF-7", "TREATMENT": "37 C", "CONDITION": "p53 status on"}}, {"GSM860200": {"SOURCE TISSUE": "human ductal breast cancer", "CELL LINE": "MCF-7", "TREATMENT": "32 C", "CONDITION": "p53 status on"}}, {"GSM860199": {"SOURCE TISSUE": "human ductal breast cancer", "CELL LINE": "MCF-7", "TREATMENT": "37 C", "CONDITION": "p53 status on"}}, {"GSM860194": {"SOURCE TISSUE": "human lobular breast cancer", "CELL LINE": "IPH-926", "TREATMENT": "32 C", "CONDITION": "p53 status on"}}, {"GSM860193": {"SOURCE TISSUE": "human lobular breast cancer", "CELL LINE": "IPH-926", "TREATMENT": "37 C", "CONDITION": "p53 status off"}}, {"GSM860194": {"SOURCE TISSUE": "human lobular breast cancer", "CELL LINE": "IPH-926", "TREATMENT": "32 C", "CONDITION": "p53 status on"}}, {"GSM860193": {"SOURCE TISSUE": "human lobular breast cancer", "CELL LINE": "IPH-926", "TREATMENT": "37 C", "CONDITION": "p53 status off"}}, {"GSM860194": {"SOURCE TISSUE": "human lobular breast cancer", "CELL LINE": "IPH-926", "TREATMENT": "32 C", "CONDITION": "p53 status on"}}, {"GSM860193": {"SOURCE TISSUE": "human lobular breast cancer", "CELL LINE": "IPH-926", "TREATMENT": "37 C", "CONDITION": "p53 status off"}}], "ownerprofile_id": "arrayexpress_sid", "platform": 4, "summary_wrapped": "The ts-p53 E285K protein is a rare p53 mutant with temperature-sensitive (ts) loss of function characteristics. In cancer cells, which...", "pubmed_id": 22945757, "geo_gse_id": "E-GEOD-35006", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 4, "sample_count": 18, "tags": ["breast", "breast cancer", "cancer", "protein"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_gds_id": "", "slug": "profiling-of-p53-responsive-genes-in-human-breast", "geo_id_plat": "E-GEOD-35006_A-AFFY-44", "name": "Profiling of p53-responsive genes in human breast cancer cells harboring endogenous ts-p53 E285K", "created": "Jul.12, 2014", "summary": "The ts-p53 E285K protein is a rare p53 mutant with temperature-sensitive (ts) loss of function characteristics. In cancer cells, which express ts-p53 E285K intriniscally, endogenous wild type p53 activity is reconstituted by appropriate cultivation temperature (permissive condition). At non-appropriate cultivation temperature (restrictive condition) this p53 mutant is inactive. The present study took advantage of this mechanism and employed IPH-926 lobular breast cancer cells and BT-474 ductal breast cancer cells, which both harbor endogenous ts-p53 E285K, for the transcriptional profiling of p53-responsive genes. This new approach eliminated the need for genetic modification or cytotoxic stimulation to achive a p53 response in the cells being investigated . Three subseqent passages of IPH-926 lobular breast cancer cells (harboring ts-p53 E285K) were seeded into two parallel culture dishes each and were allowed to adopt to restrictive and permissive condition for 24 h before analysis on Affymetrix U133 Plus 2.0 microarrays. Subsequently, this experiment was repeated with BT-474 ductal breast cancer cells (also harboring ts-p53 E285K). To gate out non-specific temperature effects, the same experiment was also performed with MCF-7 breast cancer cells (harboring wt p53). Probe sets differentially expressed at restrictive versus permissive condition in MCF-7 were considered as non-specifically regulated. These probe sets were excluded from the final statistical analysis of  IPH-926 and BT-474 expression data. response to restored p53 activity", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-35006", "species": "human", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-35006/samples/"}