ArrayExpress Uploader0mouseAtg5 flox/floxnoneAtg5 flox/floxnoneAtg5 flox/flox + LysM crenoneAtg5 flox/flox + LysM crenoneAtg5 flox/flox100U/ml of IFN-gamma for 14 hrsAtg5 flox/flox100U/ml of IFN-gamma for 14 hrsAtg5 flox/flox + LysM cre100U/ml of IFN-gamma for 14 hrsAtg5 flox/flox + LysM cre100U/ml of IFN-gamma for 14 hrs6738arrayexpress_sid6Type-I (α/β) and -II (γ) interferons (IFN), through an incompletely understood combination of redundant and unique mechanisms, are...E-GEOD-34863/profile/8773/arrayexpressuploader28bonebone marrowviral infectionDec.12, 2014Falseexpression-in-ifn-gamma-treated-control-and-atg5-mE-GEOD-34863_A-AFFY-45Expression in IFN-gamma treated control and Atg5-/- macrophagesNov.12, 2014Type-I (α/β) and -II (γ) interferons (IFN), through an incompletely understood combination of redundant and unique mechanisms, are essential for host resistance to viral infection. We report a requirement for the Atg5-Atg12/Atg16L1 autophagosome elongation complex in IFNγ-mediated control of murine norovirus in macrophages. We use microarrays to compare transcriptional changes induced in control and Atg5 deficient macrophages by IFNγ treatment. Bone marrow derived macrophages were generated from Atg5flox/flox and Atg5flox/flox+LysM cre mice and treated with media alone or 100U/ml of IFNγ for 14 hrs. RNA was extracted using Trizol (Invitrogen) and 10 µg of RNA hybridized to Affymetrix microarrays.http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-34863http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-34863/samples/