{"owner": "ArrayExpress Uploader", "pop_total": 0, "species": "mouse", "factors": [{"GSM856487": {"GENOTYPE/VARIATION": "Atg5 flox/flox", "TREATMENT": "none"}}, {"GSM856487": {"GENOTYPE/VARIATION": "Atg5 flox/flox", "TREATMENT": "none"}}, {"GSM856489": {"GENOTYPE/VARIATION": "Atg5 flox/flox + LysM cre", "TREATMENT": "none"}}, {"GSM856489": {"GENOTYPE/VARIATION": "Atg5 flox/flox + LysM cre", "TREATMENT": "none"}}, {"GSM85649": {"GENOTYPE/VARIATION": "Atg5 flox/flox", "TREATMENT": "100U/ml of IFN-gamma for 14 hrs"}}, {"GSM85649": {"GENOTYPE/VARIATION": "Atg5 flox/flox", "TREATMENT": "100U/ml of IFN-gamma for 14 hrs"}}, {"GSM856493": {"GENOTYPE/VARIATION": "Atg5 flox/flox + LysM cre", "TREATMENT": "100U/ml of IFN-gamma for 14 hrs"}}, {"GSM856493": {"GENOTYPE/VARIATION": "Atg5 flox/flox + LysM cre", "TREATMENT": "100U/ml of IFN-gamma for 14 hrs"}}], "id": 6738, "ownerprofile_id": "arrayexpress_sid", "platform": 6, "summary_wrapped": "Type-I (\u03b1/\u03b2) and -II (\u03b3) interferons (IFN), through an incompletely understood combination of redundant and unique mechanisms, are...", "geo_gse_id": "E-GEOD-34863", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 2, "sample_count": 8, "tags": ["bone", "bone marrow", "viral infection"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_gds_id": "", "slug": "expression-in-ifn-gamma-treated-control-and-atg5-m", "geo_id_plat": "E-GEOD-34863_A-AFFY-45", "name": "Expression in IFN-gamma treated control and Atg5-/- macrophages", "created": "Nov.12, 2014", "summary": "Type-I (\u03b1/\u03b2) and -II (\u03b3) interferons (IFN), through an incompletely understood combination of redundant and unique mechanisms, are essential for host resistance to viral infection. We report a requirement for the Atg5-Atg12/Atg16L1 autophagosome elongation complex in IFN\u03b3-mediated control of murine norovirus in macrophages. We use microarrays to compare transcriptional changes induced in control and Atg5 deficient macrophages by IFN\u03b3 treatment. Bone marrow derived macrophages were generated from Atg5flox/flox and Atg5flox/flox+LysM cre mice and treated with media alone or 100U/ml of IFN\u03b3 for 14 hrs. RNA was extracted using Trizol (Invitrogen) and 10 \u00b5g of RNA hybridized to Affymetrix microarrays.", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-34863", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-34863/samples/"}