{"owner": "ArrayExpress Uploader", "ownerprofile_id": "arrayexpress_sid", "species": "human", "factors": [{"GSM855583": {"PATHOLOGY": "Poorly differentiated small round cell sarcoma", "AGE": 6, "FUSION": "BCOR-CCNB3", "SEX": "female"}}, {"GSM855584": {"PATHOLOGY": "Undifferenciated small round cell malignant tumour", "AGE": 13, "FUSION": "BCOR-CCNB3", "SEX": "female"}}, {"GSM855585": {"PATHOLOGY": "Fusiform cell sarcoma", "AGE": 14, "FUSION": "BCOR-CCNB3", "SEX": "male"}}, {"GSM855586": {"PATHOLOGY": "Undifferenciated small round cell malignant tumour", "AGE": 6, "FUSION": "BCOR-CCNB3", "SEX": "female"}}, {"GSM855587": {"PATHOLOGY": "Undifferenciated small round cell malignant tumour", "AGE": 13, "FUSION": "BCOR-CCNB3", "SEX": "male"}}, {"GSM855588": {"PATHOLOGY": "Poorly differentiated small round cell sarcoma", "AGE": 12, "FUSION": "BCOR-CCNB3", "SEX": "male"}}, {"GSM855589": {"PATHOLOGY": "Poorly differentiated small round cell sarcoma", "AGE": 26, "FUSION": "BCOR-CCNB3", "SEX": "female"}}, {"GSM855590": {"PATHOLOGY": "Fusiform cell sarcoma", "AGE": 13, "FUSION": "BCOR-CCNB3", "SEX": "male"}}, {"GSM85559": {"PATHOLOGY": "Poorly differentiated small round cell sarcoma", "AGE": 10, "FUSION": "BCOR-CCNB3", "SEX": "male"}}, {"GSM855592": {"PATHOLOGY": "Poorly differentiated small round cell sarcoma", "AGE": 15, "FUSION": "BCOR-CCNB3", "SEX": "male"}}, {"GSM855593": {"PATHOLOGY": "Ewing sarcoma", "AGE": 29, "FUSION": "EWS-FLI1", "SEX": "female"}}, {"GSM855594": {"PATHOLOGY": "Ewing sarcoma", "AGE": 13, "FUSION": "EWS-FLI1", "SEX": "male"}}, {"GSM855595": {"PATHOLOGY": "Ewing sarcoma", "AGE": 17, "FUSION": "EWS-FLI1", "SEX": "female"}}, {"GSM855596": {"PATHOLOGY": "Ewing sarcoma", "AGE": 40, "FUSION": "EWS-FLI1", "SEX": "male"}}], "id": 4359, "pop_total": 0, "platform": 4, "summary_wrapped": "The identification of subtype-specific translocations has revolutionized diagnostics of sarcoma and provided new insight into...", "pubmed_id": 22387997, "geo_gse_id": "E-GEOD-34800", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 4, "sample_count": 14, "tags": ["bone", "cell", "chromosome", "ewing sarcoma", "sarcoma", "testis"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_id_plat": "E-GEOD-34800_A-AFFY-44", "slug": "a-new-subtype-of-bone-sarcoma-defined-by-bcor-ccnb", "geo_gds_id": "", "name": "A new subtype of bone sarcoma defined by BCOR-CCNB3 gene fusion", "created": "Sep.17, 2014", "summary": "The identification of subtype-specific translocations has revolutionized diagnostics of sarcoma and provided new insight into oncogenesis. We used RNA-Seq to investigate samples diagnosed as small round cell tumors of bone, possibly Ewing sarcoma, but lacking the canonical EWSR1-ETS translocation. A new fusion was observed between the BCL6 co-repressor (BCOR) and the testis specific cyclin B3 (CCNB3) genes on chromosome X. RNA-Seq results were confirmed by RT-PCR and cloning the tumor-specific genomic translocation breakpoints. 24 BCOR-CCNB3-positive tumors were identified among a series of 594 sarcomas. Gene profiling experiments indicate that BCOR-CCNB3-positive cases are biologically distinct from other sarcomas, particularly Ewing\u2019s sarcoma. Finally, we show that CCNB3 immunohistochemistry is a powerful diagnostic marker for this group of sarcoma and that over-expression of BCOR-CCNB3 or of a truncated CCNB3 activates S-phase in NIH3T3 cells. Thus the intrachromosomal X fusion described here represents a new subtype of bone sarcoma caused by a novel gene fusion mechanism. Comparison of expression profiles of 10 BCOR-CCNB3 samples (plus 4 EWS-FLI1 Ewing sarcomas samples as control) with publicly available profiles of other tumor types.", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-34800", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-34800/samples/"}