{"owner": "ArrayExpress Uploader", "pop_total": 0, "species": "human", "factors": [{"GSM854492": {"clinical information": "<=1 year biopsy - Banff: cg=0"}}, {"GSM854492": {"clinical information": "<=1 year biopsy - Banff: cg=0"}}, {"GSM854492": {"clinical information": "<=1 year biopsy - Banff: cg=0"}}, {"GSM854492": {"clinical information": "<=1 year biopsy - Banff: cg=0"}}, {"GSM854492": {"clinical information": "<=1 year biopsy - Banff: cg=0"}}, {"GSM854492": {"clinical information": "<=1 year biopsy - Banff: cg=0"}}, {"GSM854492": {"clinical information": "<=1 year biopsy - Banff: cg=0"}}, {"GSM854492": {"clinical information": "<=1 year biopsy - Banff: cg=0"}}, {"GSM854492": {"clinical information": "<=1 year biopsy - Banff: cg=0"}}, {"GSM854492": {"clinical information": "<=1 year biopsy - Banff: cg=0"}}, {"GSM854502": {"clinical information": ">=1 year biopsy - Banff: cg>0"}}, {"GSM854502": {"clinical information": ">=1 year biopsy - Banff: cg>0"}}, {"GSM854502": {"clinical information": ">=1 year biopsy - 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Banff: cg>0"}}, {"GSM854502": {"clinical information": ">=1 year biopsy - Banff: cg>0"}}, {"GSM854502": {"clinical information": ">=1 year biopsy - Banff: cg>0"}}, {"GSM854502": {"clinical information": ">=1 year biopsy - Banff: cg>0"}}, {"GSM854502": {"clinical information": ">=1 year biopsy - Banff: cg>0"}}, {"GSM854537": {"clinical information": ">=2 year biopsy - Banff: cg=0"}}, {"GSM854537": {"clinical information": ">=2 year biopsy - Banff: cg=0"}}, {"GSM854537": {"clinical information": ">=2 year biopsy - Banff: cg=0"}}, {"GSM854537": {"clinical information": ">=2 year biopsy - Banff: cg=0"}}, {"GSM854537": {"clinical information": ">=2 year biopsy - Banff: cg=0"}}, {"GSM854537": {"clinical information": ">=2 year biopsy - Banff: cg=0"}}, {"GSM854537": {"clinical information": ">=2 year biopsy - Banff: cg=0"}}, {"GSM854537": {"clinical information": ">=2 year biopsy - Banff: cg=0"}}, {"GSM854537": {"clinical information": ">=2 year biopsy - Banff: cg=0"}}, {"GSM854537": {"clinical information": ">=2 year biopsy - Banff: cg=0"}}, {"GSM854537": {"clinical information": ">=2 year biopsy - Banff: cg=0"}}], "id": 4356, "ownerprofile_id": "arrayexpress_sid", "platform": 4, "summary_wrapped": "We studied intragraft gene expression profiles of positive crossmatch (+XM) kidney transplant recipients who develop transplant...", "geo_gse_id": "E-GEOD-34748", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 1, "sample_count": 56, "tags": ["genome", "kidney"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_gds_id": "", "slug": "transcription-profiling-by-array-of-human-positive", "geo_id_plat": "E-GEOD-34748_A-AFFY-44", "name": "Transcription profiling by array of human positive crossmatch kidney transplant recipients who develop transplant gloerulopathy", "created": "Sep.17, 2014", "summary": "We studied intragraft gene expression profiles of positive crossmatch (+XM) kidney transplant recipients who develop transplant glomerulopathy (TG) and those who do not.  Whole genome microarray analysis and quantitative rt-PCR for 30 transcripts were performed on RNA from protocol renal allograft biopsies in 3 groups: 1) +XM/TG+ biopsies before and after TG; 2) +XM/NoTG; and 3) negative crossmatch kidney transplants (control).  Microarray comparisons showed few differentially expressed genes between paired biopsies from +XM/TG+ recipients before and after the diagnosis of TG.  Comparing +XM/TG+ and control groups, significantly altered expression was seen for 2,447 genes (18%) and 3,200 genes (24%) at early and late time points, respectively.  Canonical pathway analyses of differentially expressed genes showed inflammatory genes associated with innate and adaptive immune responses.  Comparing +XM/TG+ and +XM/NoTG groups, 3,718 probe sets were differentially expressed but these were over-represented in only 4 pathways.  A classic accommodation phenotype was not identified.  Using rt-PCR, the expression of inflammatory genes was significantly increased in +XM/TG+ recipients compared to control biopsies and to +XM/NoTG biopsies.  In conclusion, pre-transplant DSA results in a gene expression profile characterized by inflammation and cellular infiltration and the majority of XM+ grafts are exposed to chronic injury. We analyzed gene expression from 2 groups of positive crossmatch kidney transplant recipients (+XM/TG+ and +XM/TG-).  Patients in the +XM/TG+ group had 2 biopsies - prior to the development of transplant glomerulopathy on biopsy (Biopsy 1; cg=0; n=10) and after development of transplant glomerulopathy (Biopsy 2; cg>0; n=10).  The +XM/TG- patients had only a Biopsy 2 (cg=0; n=11).  A third patient group served as controls (n=10) and were from -XM recipients.", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-34748", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-34748/samples/"}