ArrayExpress Uploader0human<=1 year biopsy - Banff: cg=0<=1 year biopsy - Banff: cg=0<=1 year biopsy - Banff: cg=0<=1 year biopsy - Banff: cg=0<=1 year biopsy - Banff: cg=0<=1 year biopsy - Banff: cg=0<=1 year biopsy - Banff: cg=0<=1 year biopsy - Banff: cg=0<=1 year biopsy - Banff: cg=0<=1 year biopsy - Banff: cg=0>=1 year biopsy - Banff: cg>0>=1 year biopsy - Banff: cg>0>=1 year biopsy - Banff: cg>0>=1 year biopsy - Banff: cg>0>=1 year biopsy - Banff: cg>0>=1 year biopsy - Banff: cg>0>=1 year biopsy - Banff: cg>0>=1 year biopsy - Banff: cg>0>=1 year biopsy - Banff: cg>0>=1 year biopsy - Banff: cg>0<=1 year biopsy - Banff: cg=0<=1 year biopsy - Banff: cg=0<=1 year biopsy - Banff: cg=0<=1 year biopsy - Banff: cg=0<=1 year biopsy - Banff: cg=0<=1 year biopsy - Banff: cg=0<=1 year biopsy - Banff: cg=0<=1 year biopsy - Banff: cg=0<=1 year biopsy - Banff: cg=0<=1 year biopsy - Banff: cg=0>=1 year biopsy - Banff: cg=0>=1 year biopsy - Banff: cg=0>=1 year biopsy - Banff: cg=0>=1 year biopsy - Banff: cg=0>=1 year biopsy - Banff: cg=0>=1 year biopsy - Banff: cg=0>=1 year biopsy - Banff: cg=0>=1 year biopsy - Banff: cg=0>=1 year biopsy - Banff: cg=0>=1 year biopsy - Banff: cg=0>=1 year biopsy - Banff: cg>0>=1 year biopsy - Banff: cg>0>=1 year biopsy - Banff: cg>0>=1 year biopsy - Banff: cg>0>=1 year biopsy - Banff: cg>0>=2 year biopsy - Banff: cg=0>=2 year biopsy - Banff: cg=0>=2 year biopsy - Banff: cg=0>=2 year biopsy - Banff: cg=0>=2 year biopsy - Banff: cg=0>=2 year biopsy - Banff: cg=0>=2 year biopsy - Banff: cg=0>=2 year biopsy - Banff: cg=0>=2 year biopsy - Banff: cg=0>=2 year biopsy - Banff: cg=0>=2 year biopsy - Banff: cg=04356arrayexpress_sid4We studied intragraft gene expression profiles of positive crossmatch (+XM) kidney transplant recipients who develop transplant...E-GEOD-34748/profile/8773/arrayexpressuploader156genomekidneyDec.12, 2014Falsetranscription-profiling-by-array-of-human-positiveE-GEOD-34748_A-AFFY-44Transcription profiling by array of human positive crossmatch kidney transplant recipients who develop transplant gloerulopathySep.17, 2014We studied intragraft gene expression profiles of positive crossmatch (+XM) kidney transplant recipients who develop transplant glomerulopathy (TG) and those who do not. Whole genome microarray analysis and quantitative rt-PCR for 30 transcripts were performed on RNA from protocol renal allograft biopsies in 3 groups: 1) +XM/TG+ biopsies before and after TG; 2) +XM/NoTG; and 3) negative crossmatch kidney transplants (control). Microarray comparisons showed few differentially expressed genes between paired biopsies from +XM/TG+ recipients before and after the diagnosis of TG. Comparing +XM/TG+ and control groups, significantly altered expression was seen for 2,447 genes (18%) and 3,200 genes (24%) at early and late time points, respectively. Canonical pathway analyses of differentially expressed genes showed inflammatory genes associated with innate and adaptive immune responses. Comparing +XM/TG+ and +XM/NoTG groups, 3,718 probe sets were differentially expressed but these were over-represented in only 4 pathways. A classic accommodation phenotype was not identified. Using rt-PCR, the expression of inflammatory genes was significantly increased in +XM/TG+ recipients compared to control biopsies and to +XM/NoTG biopsies. In conclusion, pre-transplant DSA results in a gene expression profile characterized by inflammation and cellular infiltration and the majority of XM+ grafts are exposed to chronic injury. We analyzed gene expression from 2 groups of positive crossmatch kidney transplant recipients (+XM/TG+ and +XM/TG-). Patients in the +XM/TG+ group had 2 biopsies - prior to the development of transplant glomerulopathy on biopsy (Biopsy 1; cg=0; n=10) and after development of transplant glomerulopathy (Biopsy 2; cg>0; n=10). The +XM/TG- patients had only a Biopsy 2 (cg=0; n=11). A third patient group served as controls (n=10) and were from -XM recipients.http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-34748http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-34748/samples/