ArrayExpress Uploaderarrayexpress_sidhumanpatient 11papillary thyroid carcinomafemalepatient 50papillary thyroid carcinomamalepatient 69papillary thyroid carcinomamalepatient 11normalfemalepatient 14normalmalepatient 26normalmalepatient 50normalmalepatient 67normalfemalepatient 69normalmalepatient 71normalmalepatient 77normalfemalepatient 86normalfemalepatient 14papillary thyroid carcinomamalepatient 26papillary thyroid carcinomamalepatient 67papillary thyroid carcinomafemalepatient 71papillary thyroid carcinomamalepatient 77papillary thyroid carcinomafemale435404We show that numerous miRNAs are transcriptionally up-regulated in papillary thyroid carcinoma (PTC) tumors compared with unaffected...16365291E-GEOD-3467/profile/8773/arrayexpressuploader317carcinomapapillary thyroid carcinomaproteinthyroid carcinomaDec.12, 2014FalseE-GEOD-3467_A-AFFY-44transcription-profiling-of-micro-rna-genes-in-humaTranscription profiling of micro-RNA genes in human papillary thyroid carcinomaSep.17, 2014We show that numerous miRNAs are transcriptionally up-regulated in papillary thyroid carcinoma (PTC) tumors compared with unaffected thyroid tissue. Among the predicted target genes of the three most upregulated miRNAs (miRs 221, 222 and 146b), only less than 15% showed significant downexpression in transcript level between tumor and unaffected tissue. The KIT gene which is known to be downregulated by miRNAs 221 and 222 displayed dramatic loss of transcript and protein in those tumors that had abundant mir-221, mir-222, and mir-146b transcript. Experiment Overall Design: Total RNA was extracted from paired tumor and normal thyroid tissues from 9 PTC patients. The same set samples were applied to Custom miRNA microarray chips (OSU_CCC version 2.0) and Affymetrix HG-U133 plus 2 chips.http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-3467http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-3467/samples/