{"owner": "ArrayExpress Uploader", "pop_total": 0, "species": "mouse", "factors": [{"GSM851629": {"TREATMENT": "ethanol (EtOH)"}}, {"GSM851630": {"TREATMENT": "4-hydroxytamoxifen (4OHT)"}}, {"GSM851629": {"TREATMENT": "ethanol (EtOH)"}}, {"GSM851630": {"TREATMENT": "4-hydroxytamoxifen (4OHT)"}}], "id": 8277, "ownerprofile_id": "arrayexpress_sid", "platform": 7, "summary_wrapped": "We performed microarray analysis to examine the differential gene expression profiles between Prdm1 (Blimp-1)-deleted and control...", "geo_gse_id": "E-GEOD-34586", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 1, "sample_count": 4, "tags": [], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_gds_id": "", "slug": "comparison-of-the-transcripts-in-control-and-blimp", "geo_id_plat": "E-GEOD-34586_A-AFFY-23", "name": "Comparison of the transcripts in control and Blimp-1-deficient keratinocytes", "created": "Nov.23, 2014", "summary": "We performed microarray analysis to examine the differential gene expression profiles between Prdm1 (Blimp-1)-deleted and control keratinocytes. Keratinocytes isolated from Prdm1-floxed K5-CreER positive (CKO) mice were cultured in the presence of 4OHT to induce deletion of the Prdm1 allele in vitro. Prdm1-floxed K5-CreER positive (CKO) keratinocytes treated with the ethanol solvent control (EtOH) or Prdm1-floxed K5-CreER negative (control) keratinocytes treated with 4OHT or EtOH served as controls. Microarray analyses revealed that there were 93 genes up-regulated and 109 genes down-regulated by more than 2-fold in the CKO + 4OHT group in comparison with the CKO + EtOH, Ctrl + 4OHT or Ctrl + EtOH groups. Several corneocytes-related genes, including Rptn, Lce1f, Krt1 and Lce1d, are significantly down-regulated and several cytokines/chemokines, including Cxcl1, Cxcl2, Cxcl5 and Il24, are significantly up-regulated upon the deletion of Prdm1 in vitro. We analyzed the transcription profiles in control and Blimp-1-deficient keratinocytes using the GeneChip\u00ae Mouse Expression Array 430A.", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-34586", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-34586/samples/"}