ArrayExpress Uploader0humanCD16negCD16negCD16negCD16posCD16posCD16posCD1c+CD1c+CD1c+4379arrayexpress_sid4In this study gene expression of CD1c+ CD19- blood dendritic cells from healthy subjects were investigated. Keywords: expression...E-GEOD-34515/profile/8773/arrayexpressuploader19celldendriticDec.12, 2014Falsegene-expression-profiles-of-human-cd1c-cd19-bloodE-GEOD-34515_A-AFFY-44Gene expression profiles of human CD1c+ CD19- blood dendritic cells [human data]Sep.19, 2014In this study gene expression of CD1c+ CD19- blood dendritic cells from healthy subjects were investigated. Keywords: expression profiling by array Blood DCs (CD1c+ CD19-) were isolated from venous heparinized blood of three apparently healthy human volunteers using the CD1c+ (BDCA-1) dendritic cell isolation kit from Miltenyi according to the manufacturer’s instructions. Purity over 90% (95.8±3%) was determined by FACS analysis. RNA was isolated and 1 µg was labeled using the Affymetrix One-Cycle Target Labeling Kit and the biotinylated cRNA used to perform Affymetrix U133 Plus 2.0 expression arrays.http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-34515http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-34515/samples/