{"owner": "ArrayExpress Uploader", "pop_total": 0, "species": "human", "factors": [{"GSM84944": {"TRANSFECTED": "miR-376a*A (72 h)"}}, {"GSM84944": {"TRANSFECTED": "miR-376a*A (72 h)"}}, {"GSM84944": {"TRANSFECTED": "miR-376a*A (72 h)"}}, {"GSM849444": {"TRANSFECTED": "miR-376a*G (72 h)"}}, {"GSM849444": {"TRANSFECTED": "miR-376a*G (72 h)"}}, {"GSM849444": {"TRANSFECTED": "miR-376a*G (72 h)"}}, {"GSM849447": {"TRANSFECTED": "control miRNA (72 h)"}}, {"GSM849447": {"TRANSFECTED": "control miRNA (72 h)"}}, {"GSM849447": {"TRANSFECTED": "control miRNA (72 h)"}}], "id": 4376, "ownerprofile_id": "arrayexpress_sid", "platform": 4, "summary_wrapped": "Several biological pathways can be under the regulation of miRNAs. These pathways can be indentified by the enforced expression of a...", "geo_gse_id": "E-GEOD-34456", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 1, "sample_count": 9, "tags": [], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_gds_id": "", "slug": "expression-data-from-transfection-of-u87-glioma-2", "geo_id_plat": "E-GEOD-34456_A-AFFY-44", "name": "Expression data from transfection of U87 glioma cells with miR-376a* for 72 hours", "created": "Sep.19, 2014", "summary": "Several biological pathways can be under the regulation of miRNAs. These pathways can be indentified by the enforced expression of a miRNA and analysing the expression data for enrichment of specific pathways represented among the genes differentially expressed upon miRNA overexpression. We transfected unedited miR-376a* (376a*A) and edited miR-376a* (376a*G) into U87 cells to identify differentially regulated transcripts. List of differentially expressed genes were subject to pathway enrichment analysis to identify specific biological processes regulated by each of miR-376a*A and miR-376a*G. U87 cells (100,000) were transfected with 50 nM of miR-376a*A, miR-376a*G or miR-control. Following incubation for 72 hours, total RNA was isolated from transfected cells. For each (miR-376a*A or miR-376a*G), genes differentially expressed upon miRNA transfection were determined based on comparison with miR-control-transfected samples. Biological triplicates were used for each sample type.", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-34456", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-34456/samples/"}