{"owner": "ArrayExpress Uploader", "pop_total": 0, "species": "human", "factors": [{"GSM849423": {"TRANSFECTED": "miR-376a*A (24 h)"}}, {"GSM849423": {"TRANSFECTED": "miR-376a*A (24 h)"}}, {"GSM849423": {"TRANSFECTED": "miR-376a*A (24 h)"}}, {"GSM849426": {"TRANSFECTED": "miR-376a*G (24 h)"}}, {"GSM849426": {"TRANSFECTED": "miR-376a*G (24 h)"}}, {"GSM849426": {"TRANSFECTED": "miR-376a*G (24 h)"}}, {"GSM849429": {"TRANSFECTED": "control miRNA (24 h)"}}, {"GSM849429": {"TRANSFECTED": "control miRNA (24 h)"}}, {"GSM849429": {"TRANSFECTED": "control miRNA (24 h)"}}], "id": 4374, "ownerprofile_id": "arrayexpress_sid", "platform": 4, "summary_wrapped": "Enforced expression of miRNAs in cells leads to down-regulation of several mRNAs which harbour binding sites in their 3'UTRs for the...", "geo_gse_id": "E-GEOD-34454", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 1, "sample_count": 9, "tags": [], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_gds_id": "", "slug": "expression-data-from-transfection-of-sw1783-glioma", "geo_id_plat": "E-GEOD-34454_A-AFFY-44", "name": "Expression data from transfection of SW1783 glioma cells with microRNA-376a* for 24 hours", "created": "Sep.19, 2014", "summary": "Enforced expression of miRNAs in cells leads to down-regulation of several mRNAs which harbour binding sites in their 3'UTRs for the overexpressed miRNA and represent potential target genes of the miRNA We transfected unedited miR-376a* (376a*A) and edited miR-376a* (376a*G) into SW1783 cells to identify transcripts down-regulated by each in comparison to control  miRNA transfection. The aim was to identify target genes subject to direct regulation by miR-376a*A and by miR-376a*G. SW1783 cells (100,000) were transfected with 50 nM of miR-376a*A, miR-376a*G or miR-control. Following incubation for 24 hours, total RNA was isolated from transfected cells. For each (miR-376a*A or miR-376a*G), genes differentially expressed upon miRNA transfection were determined based on comparison with miR-control-transfected samples. Biological triplicates were used for each sample type.", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-34454", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-34454/samples/"}