{"owner": "ArrayExpress Uploader", "pop_total": 0, "id": 8593, "factors": [{"GSE3433GSM77380": {"TREATMENT": "low fat diet", "STRAINORLINE": "A/J"}}, {"GSE3433GSM77381": {"TREATMENT": "high fat diet", "STRAINORLINE": "A/J"}}, {"GSE3433GSM77382": {"TREATMENT": "low fat diet", "STRAINORLINE": "C57BL/6J "}}, {"GSE3433GSM77383": {"TREATMENT": "high fat diet", "STRAINORLINE": "C57BL/6J "}}], "ownerprofile_id": "arrayexpress_sid", "platform": 8, "summary_wrapped": "The effects of high-fat (HF) feeding on the gene expression in the small intestine were examined using obesity-resistant A/J mice and...", "pubmed_id": 16822957, "geo_gse_id": "E-GEOD-3433", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 2, "sample_count": 4, "tags": ["body", "intestine", "lipid", "obesity", "small intestine"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_gds_id": "", "slug": "transcription-profiling-of-mouse-strains-aj-versus", "geo_id_plat": "E-GEOD-3433_A-AFFY-36", "name": "Transcription profiling of mouse strains A/J versus C57BL/6J during diet-induced obesity", "created": "Nov.24, 2014", "summary": "The effects of high-fat (HF) feeding on the gene expression in the small intestine were examined using obesity-resistant A/J mice and obesity-sensitive C57BL/6J (B6) mice. Both strains of mice were maintained on low-fat (LF, 5% fat) or HF (30% fat) diets for two weeks. Oligonucleotide microarray and semi-quantitative RT-PCR analysis revealed that lipid metabolism-related genes including mitochondrial acyl-CoA thioesterase 1, pyruvate dehydrogenase kinase 4, and NADP+-dependent cytosolic malic enzyme were up-regulated by more than 2-fold in both strains of mice. The up-regulated gene expression levels were significantly higher in A/J mice than in B6 mice, suggesting the active lipid metabolism in the small intestine of A/J mice.  Experiment Overall Design: Mice were maintained on the LF or HF diet for two weeks. For each group (n=6), pooled total RNA from two mice of average body weight was used for the analysis.", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-3433", "species": "mouse", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-3433/samples/"}